Rhein

Name: Rhein
Brand: Tocris Bioscience
SKU: 6867

Tocris Bioscience, part of Bio-Techne | Catalog # 6867

COA / SDS

FTO (mRNA N6-methyladenine demethylase) inhibitor; also inhibits ALKBH2 and ALKBH3

Discontinued Product

6867 has been discontinued. View all Other Oxidase/Oxygenase Compounds products.

Key Product Details

Description:	FTO (mRNA N⁶-methyladenine demethylase) inhibitor; also inhibits ALKBH2 and ALKBH3
Chemical Name:	9,10-Dihydro-4,5-dihydroxy-9,10-dioxo-2-anthracenecarboxylic acid
Purity:	≥98% (HPLC)
Molecular Weight:	284.22

View Full Technical Details for Rhein

Technical Data
Citations

Biological Activity for Rhein

Rhein is a FTO (mRNA N⁶-methyladenine demethylase) inhibitor (IC₅₀ = 1.2 - 21 μM; K_i = 1.8 μM). Competitively binds enzyme at active site preventing binding of N⁶-methyladenosine (m⁶A) substrate and resulting in accumulation of DNA damage in cells. Also inhibits ALKBH3 and ALKBH2 (IC₅₀values are 5.3 and 9.1 μM, respectively). Sensitizes glioblastoma cells to alkylating agents. Active in vivo.

Technical Data for Rhein

M. Wt	284.22
Formula	C₁₅H₈O₆
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	478-43-3
PubChem ID	10168
InChi Key	FCDLCPWAQCPTKC-UHFFFAOYSA-N
Smiles	OC(=O)C1=CC2=C(C(O)=C1)C(=O)C1=C(O)C=CC=C1C2=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for Rhein

References are publications that support the biological activity of the product.

Svensen and Jaffrey Fluorescent RNA aptamers as a tool to study RNA-modifying enzymes. Cell.Chem.Biol. 2016 PMID: 26877022
Li Rhein inhibits AlkB repair enzymes and sensitizes cells to methylated DNA damage. J.Biol.Chem. 2016 PMID: 27015802
Chen Development of cell-active N⁶-methyladenosine RNA demethylase FTO inhibitor. J.Am.Chem.Soc. 2012 PMID: 23045983

Rhein

Tocris Bioscience, part of Bio-Techne | Catalog # 6867

Key Product Details

Biological Activity for Rhein

Technical Data for Rhein

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

References for Rhein

Product Documents for Rhein

COA

Certificate of Analysis

SDS

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