RAGE 229

Name: RAGE 229
Brand: Tocris Bioscience
SKU: 7701

Tocris Bioscience, part of Bio-Techne | Catalog # 7701

COA / SDS

ctRAGE-DIAPH1 interaction antagonist; reduces inflammatory signaling in diabetic mice

Key Product Details

Description:	ctRAGE-DIAPH1 interaction antagonist; reduces inflammatory signaling in diabetic mice
Chemical Name:	N-[4-[7-Cyano-4-(4-morpholinylmethyl)-2-quinolinyl]phenyl]acetamide
Purity:	≥98% (HPLC)
Molecular Weight:	386.45

View Full Technical Details for RAGE 229

Technical Data
Citations

Biological Activity for RAGE 229

RAGE 229 is an antagonist of the interaction between the cytoplasmic tail of the receptor for advanced glycation end products (ctRAGE) and the formin, Diaphanous-1 (DIAPH1) (K_D for binding to ctRAGE = 2 nM). RAGE 229 inhibits the migration of human aortic smooth muscle cells in an in vitro wound healing assay (IC₅₀ = 120 nM). RAGE 229 reduces short- and long-term complications of diabetes in mouse models, without lowering blood glucose concentrations. RAGE 229 also reduces plasma concentrations of TNF-α, IL-6, and CCL2/JE-MCP1 and attenuates inflammatory signaling in diabetic mice.

Licensing Information

Sold under license from New York University

Technical Data for RAGE 229

M. Wt	386.45
Formula	C₂₃H₂₂N₄O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2143072-85-7
PubChem ID	132020228
InChi Key	VDYYVNHJRNDKDO-UHFFFAOYSA-N
Smiles	N#CC=1C=CC=2C(=NC(=CC2CN3CCOCC3)C=4C=CC(=CC4)NC(=O)C)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for RAGE 229

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	38.65	100

Preparing Stock Solutions for RAGE 229

The following data is based on the product molecular weight 386.45.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.59 mL	12.94 mL	25.88 mL
5 mM	0.52 mL	2.59 mL	5.18 mL
10 mM	0.26 mL	1.29 mL	2.59 mL
50 mM	0.05 mL	0.26 mL	0.52 mL

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References for RAGE 229

References are publications that support the biological activity of the product.

Manigrasso Small-molecule antagonism of the interaction of the RAGE cytoplasmic domain with DIAPH1 reduces diabetic complications in mice. Sci.Transl.Med. 2021 PMID: 34818060