PYR 41

Name: PYR 41
Brand: Tocris Bioscience
SKU: 2978
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 2978

(1)

Citations (30)

COA / SDS

Ubiquitin-activating enzyme (E1) inhibitor

Key Product Details

Description:	Ubiquitin-activating enzyme (E1) inhibitor
Chemical Name:	4-[4-[(5-Nitro-2-furanyl)methylene]-3,5-dioxo-1-pyrazolidinyl]benzoic acid ethyl ester
Purity:	≥98% (HPLC)
Molecular Weight:	371.3

View Full Technical Details for PYR 41

Technical Data
Citations / Reviews (1)

Biological Activity for PYR 41

PYR 41 is a cell-permeable, irreversible ubiquitin-activating enzyme (E1) inhibitor (IC₅₀ < 10 μM). Blocks ubiquitination and prevents ubiquitin-mediated proteasomal degradation. Inhibits NF-κB activation, blocks degradation of p53, increases p21 levels and induces apoptosis in vitro. Also causes an increase in sumoylation of proteins.

Technical Data for PYR 41

M. Wt	371.3
Formula	C₁₇H₁₃N₃O₇
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	418805-02-4
PubChem ID	2856906
InChi Key	ARGIPZKQJGFSGQ-UHFFFAOYSA-N
Smiles	CCOC(=O)C1=CC=C(C=C1)N1NC(=O)C(=CC2=CC=C(O2)[N+]([O-])=O)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PYR 41

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	37.13	100

Preparing Stock Solutions for PYR 41

The following data is based on the product molecular weight 371.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.69 mL	13.47 mL	26.93 mL
5 mM	0.54 mL	2.69 mL	5.39 mL
10 mM	0.27 mL	1.35 mL	2.69 mL
50 mM	0.05 mL	0.27 mL	0.54 mL

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Reconstitution Calculator

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References for PYR 41

References are publications that support the biological activity of the product.

Mi Cancer preventative isothiocyanates induce selective degradation of cellular α- and β-tubulins by proteasomes. J.Biol.Chem. 2009 PMID: 19339240
Brahemi Homology modelling of human E1 ubiquitin activating enzyme. Lett.Drug Des.Discov. 2010 PMID: 20396627
Yang Inhibitors of ubiquitin-activating enzyme (E1), a new class of potential cancer therapeutics. Cancer Res. 2007 PMID: 17909057