preQ1-biotin
Tocris Bioscience, part of Bio-Techne | Catalog # 7804
PreQ1 with biotin handle for RNA labeling and purification
Key Product Details
Description
PreQ1 with biotin handle for RNA labeling and purification
Product Description
preQ1-biotin is a modified analog of the natural substrate prequeosine1 (preQ1) and is used for RNA-TAG (transglycosylation at guanosine) and DNA-TAG. preQ1-biotin enables affinity tagging and pull-down of specific RNAs that have been modified selectively by E. coli tRNA guanine transglycosylase (TGT). preQ1-biotin is incorporated site-specifically and covalently into RNAs containing a short harpin nucleotide recognition motif. The guanine in a UGU recognition element is exchanged with the preQ1-biotin substrate.Plasmids for expression of the E.coli TGT enzyme (#138201) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end (#138209) are available from Addgene.
Scientific Data Images for preQ1-biotin
E. coli TGT irreversibly exchanges a specific guanine in a DNA hairpin with preQ1-biotin.
Schematic representation of preQ1-bitoin insertion into DNA.
E. coli TGT irreversibly exchanges a specific guanine in a DNA hairpin with preQ1-biotin.
Urea-PAGE gel shift analysis of preQ1-biotin insertion. Modification is evidenced by an upward shift of the product band due to molecular weight increase. Lane 1 is unmodified DNA; lane 2 is preQ1-biotin modified DNA.Licensing Information
Sold under license from The Regents of the University of California.Product Specifications
Molecular Weight
504.65
Formula
C23H36N8O3S
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
N-(6-(((2-Amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl)amino)hexyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
CAS Number
2971850-25-4
InChI Key
APUALFAJXKNNBF-BXWFABGCSA-N
SMILES
O=C1C2=C(NC=C2CNCCCCCCNC(CCCC[C@H]3[C@]4([H])[C@](CS3)(NC(N4)=O)[H])=O)N=C(N)N1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 10.09 | 20 | |
| Ethanol | 2.52 | 5 |
Preparing Stock Solutions
for preQ1-biotin
The following data is based on the product molecular weight 504.65
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 9.91 mL | 49.54 mL | 99.08 mL |
| 1 mM | 1.98 mL | 9.91 mL | 19.82 mL |
| 2 mM | 0.99 mL | 4.95 mL | 9.91 mL |
| 10 mM | 0.20 mL | 0.99 mL | 1.98 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Tota and Devaraj Site-specific covalent labeling of DNA substrates by an RNA transglycosylase. J.Am.Chem.Soc 2023 PMID: 36988146
- Busby Enzymatic RNA biotinylation for affinity purification and identification of RNA-protein interactions. ACS Chem.Biol. 2020 PMID: 32706237
Product Specific Notices for preQ1-biotin
For research use only
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