(+)-JQ1 maleimide
Tocris Bioscience, part of Bio-Techne | Catalog # 7576
JQ1-maleimide functionalized probe; induces proteasomal degradation of BRD4
Key Product Details
Description
JQ1-maleimide functionalized probe; induces proteasomal degradation of BRD4
Product Description
(+)-JQ1 maleimide is a probe that comprises a cysteine-reactive maleimide connected to the BRD4 ligand, JQ1, via a PEG2 linker. It can be used in the COFFEE method (COvalent Functionalization Followed by E3 Electroporation) in which (+)-JQ1 maleimide is covalently attached to VHL followed by electroporation into live cells. The E3 ligase forms a complex with BRD4 intracellularly and induces its degradation.Product Specifications
Molecular Weight
724.27
Formula
C35H42ClN7O6S
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(S)-N-(2-(2-(2-(2-(4-(4-Chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetamido)ethoxy)ethoxy)ethyl)-6-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)hexanamide
InChI Key
SGQDAIFBYPRIDQ-MHZLTWQESA-N
SMILES
ClC1=CC=C(C2=N[C@@H](CC(NCCOCCOCCNC(CCCCCN3C(C=CC3=O)=O)=O)=O)C4=NN=C(C)N4C5=C2C(C)=C(C)S5)C=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 72.43 | 100 |
Preparing Stock Solutions
for (+)-JQ1 maleimide
The following data is based on the product molecular weight 724.27
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.38 mL | 6.90 mL | 13.81 mL |
| 5 mM | 0.28 mL | 1.38 mL | 2.76 mL |
| 10 mM | 0.14 mL | 0.69 mL | 1.38 mL |
| 50 mM | 0.03 mL | 0.14 mL | 0.28 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Pinch A strategy to assess the cellular activity of E3 ligase components against neo-substrates using electrophilic probes. Cell Chem.Biol. 2022 PMID: 34499862
Product Specific Notices for (+)-JQ1 maleimide
For research use only
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