PF 06409577

Name: PF 06409577
Brand: Tocris Bioscience
SKU: 6114

Tocris Bioscience, part of Bio-Techne | Catalog # 6114

Citations (6)

COA / SDS

Potent and selective allosteric activator of AMPK

Key Product Details

Description:	Potent and selective allosteric activator of AMPK
Chemical Name:	6-Chloro-5-[4-(1-hydroxycyclobutyl)phenyl]-1H-indole-3-carboxylic acid
Purity:	≥98% (HPLC)
Molecular Weight:	341.79

View Full Technical Details for PF 06409577

Technical Data
Citations

Biological Activity for PF 06409577

PF 06409577 is a potent and selective allosteric activator of AMPK (EC₅₀ values are 7 and >40000 nM for AMPKα1β1γ1 and AMPKα1β2γ1, respectively). Exhibits minimal off-target effects on a panel of receptors, ion channels, PDEs and kinases. Improves kidney function and reduces proteinuria in a preclinical model of diabetic nephropathy. Orally bioavailable.

Licensing Information

Sold for research purposes under agreement from Pfizer Inc.

Compound Libraries for PF 06409577

PF 06409577 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for PF 06409577

M. Wt	341.79
Formula	C₁₉H₁₆ClNO₃
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	1467057-23-3
PubChem ID	71748255
InChi Key	FHQXLWCFSUSXBF-UHFFFAOYSA-N
Smiles	O=C(O)C1=CNC2=CC(Cl)=C(C3=CC=C(C4(CCC4)O)C=C3)C=C21

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PF 06409577

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	34.18	100
	ethanol	6.84	20

Preparing Stock Solutions for PF 06409577

The following data is based on the product molecular weight 341.79.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.93 mL	14.63 mL	29.26 mL
5 mM	0.59 mL	2.93 mL	5.85 mL
10 mM	0.29 mL	1.46 mL	2.93 mL
50 mM	0.06 mL	0.29 mL	0.59 mL

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References for PF 06409577

References are publications that support the biological activity of the product.

Salatto Selective activation of AMPK β1-containing isoforms improves kidney function in a rat model of diabetic nephropathy. J.Pharmacol.Exp.Ther. 2017 PMID: 28289077
Cameron Discovery and preclinical characterization of 6-Chloro-5-[4-(1-hydroxycyclobutyl)phenyl]-1H-indole-3-carboxylic Acid (PF-06409577), a direct activator of adenosine monophosphate-activated protein kinase (AMPK), for the potential treatment of diab J.Med.Chem. 2016 PMID: 27490827