PARPYnD
Tocris Bioscience, part of Bio-Techne | Catalog # 7410
PARP inhibitor; photoaffinity probe
Key Product Details
Description
PARP inhibitor; photoaffinity probe
Product Description
PARPYnD is a potent photoaffinity probe (AfBP) for poly(ADP-ribose) polymerase (PARP) (IC50 values for PARP2, PARP1 and PARP6 are 6, 38 and 230 nM, respectively). Can be used to fluorescently label PARP1 and PARP2 in cells when an azide functionalized probe is attached via copper catalysed click-chemistry. PARPYnD enriches recombinant PARP6 spiked into cellular lysates and inhibits PARP6 in cell-free assays; does not label PARP6 in intact cells. Photocrosslinking can be performed by irradiating at 365 nm.Product Specifications
Molecular Weight
613.68
Formula
C34H31N9O3
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
3-(3-Butyn-1-yl)-N-[5-cyano-6-[4-[3-[(3,4-dihydro-4-oxo-1-phthalazinyl)methyl]benzoyl]-1-piperazinyl]-3-pyridinyl]-3H-diazirine-3-propanamide
CAS Number
2561483-27-8
InChI Key
ZYWZRMXOMDKHKX-UHFFFAOYSA-N
SMILES
O=C(NC1=CN=C(C(C#N)=C1)N2CCN(C(C3=CC(CC4=NNC(C5=CC=CC=C45)=O)=CC=C3)=O)CC2)CCC6(N=N6)CCC#C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 61.37 | 100 |
Preparing Stock Solutions
for PARPYnD
The following data is based on the product molecular weight 613.68
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.63 mL | 8.15 mL | 16.30 mL |
| 5 mM | 0.33 mL | 1.63 mL | 3.26 mL |
| 10 mM | 0.16 mL | 0.81 mL | 1.63 mL |
| 50 mM | 0.03 mL | 0.16 mL | 0.33 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Howard Structure-guided design and in-cell target profiling of a cell-active target engagement probe for PARP inhibitors. ACS Chem.Biol. 2020 PMID: 32017532
Product Specific Notices for PARPYnD
For research use only
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