para-Nitroblebbistatin
Tocris Bioscience, part of Bio-Techne | Catalog # 8804
Selective inhibitor of myosin II ATPase activity. Non-cytotoxic version of (±)-Blebbistatin (Cat. No. 1760)
Key Product Details
Description
Selective inhibitor of myosin II ATPase activity. Non-cytotoxic version of (±)-Blebbistatin (Cat. No. 1760)
Product Description
para-Nitroblebbistatin is a selective inhibitor of Myosin II ATPase activity (IC50 = 15 μM). It is a non-fluorescent, photostable, non-cytotoxic and non-phototoxic derivative of (±)-Blebbistatin (Cat. No. 1760).(S)-(-)-Blebbistatin (Cat. No. 1852) also available.
Product Specifications
Molecular Weight
337.34
Formula
C18H15N3O4
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(3aS)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-(4-nitrophenyl)-4H-pyrrolo[2,3-b]quinolin-4-one
CAS Number
1621326-32-6
PubChem ID
102361739
InChI Key
KAUXNLHXGQGFOS-GOSISDBHSA-N
SMILES
O[C@@]12C(N(C3=CC=C(C=C3)[N+]([O-])=O)CC2)=NC4=CC=C(C)C=C4C1=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 16.87 | 50 |
Preparing Stock Solutions
for para-Nitroblebbistatin
The following data is based on the product molecular weight 337.34
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 5.93 mL | 29.64 mL | 59.29 mL |
| 2.5 mM | 1.19 mL | 5.93 mL | 11.86 mL |
| 5 mM | 0.59 mL | 2.96 mL | 5.93 mL |
| 25 mM | 0.12 mL | 0.59 mL | 1.19 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Képiró para-Nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitor Angew.Chem Int.Ed.Engl. 2011 PMID: 24954740
- Värkuti A highly soluble, non-phototoxic, non-fluorescent blebbistatin derivative. Sci.Rep. 2016 PMID: 27241904
- Gollapudi Two classes of myosin inhibitors, para-nitroblebbistatin and mavacamten, stabilize β-cardiac myosin in different structural and functional states. J.Mol.Biol. 2021 PMID: 34627791
Product Specific Notices for para-Nitroblebbistatin
For research use only
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