P 22077

Name: P 22077
Brand: Tocris Bioscience
SKU: 4485
Rating: 4 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4485

(2)

Citations (10)

COA / SDS

USP7 inhibitor

Key Product Details

Description:	USP7 inhibitor
Chemical Name:	1-[5-[(2,4-Difluorophenyl)thio]-4-nitro-2-thienyl]-ethanone
Purity:	≥98% (HPLC)
Molecular Weight:	315.32

View Full Technical Details for P 22077

Technical Data
Citations / Reviews (2)

Biological Activity for P 22077

P 22077 is an inhibitor of ubiquitin-specific protease (USP) 7 (EC₅₀ = 8.6 μM); also inhibits the closely related deubiquitinase (DUB) USP47. Demonstrates downstream inhibition of HDM2 and claspin in vitro. Inhibits USP7-mediated p53 deubiquitination. Blocks deubiquitination of Tip60 (KAT5) histone lysine acetyltransferase.

Compound Libraries for P 22077

P 22077 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for P 22077

M. Wt	315.32
Formula	C₁₂H₇F₂NO₃S₂
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1247819-59-5
PubChem ID	46931953
InChi Key	RMAMGGNACJHXHO-UHFFFAOYSA-N
Smiles	CC(C1=CC([N+]([O-])=O)=C(SC2=CC=C(F)C=C2F)S1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for P 22077

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	31.53	100

Preparing Stock Solutions for P 22077

The following data is based on the product molecular weight 315.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.17 mL	15.86 mL	31.71 mL
5 mM	0.63 mL	3.17 mL	6.34 mL
10 mM	0.32 mL	1.59 mL	3.17 mL
50 mM	0.06 mL	0.32 mL	0.63 mL

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References for P 22077

References are publications that support the biological activity of the product.

Dar Deubiquitination of Tip60 by USP7 determines the activity of the p53-dependent apoptotic pathway. Mol.Cell Biol. 2013 PMID: 23775119
Tian Characterization of selective ubiquitin and ubiquitin-like protease inhibitors using a fluorescence-based multiplex assay format. Assay Drug Dev.Technol. 2011 PMID: 21133675
Altun Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. Chem.Biol. 2011 PMID: 22118674
Loch Deubiquitylase, deSUMOylase, and deISGylase activity microarrays for assay of substrate preference and functional modifiers. Mol.Cell.Proteomics 2011 PMID: 20956615