NHI 2

Name: NHI 2
Brand: Tocris Bioscience
SKU: 5363

Tocris Bioscience, part of Bio-Techne | Catalog # 5363

Citations (3)

COA / SDS

LDHA inhibitor

Key Product Details

Description:	LDHA inhibitor
Chemical Name:	Methyl 1-hydroxy-6-phenyl-4-(trifluoromethyl)-1H-indole-2-carboxylate
Purity:	≥98% (HPLC)
Molecular Weight:	335.28

View Full Technical Details for NHI 2

Technical Data
Citations

Biological Activity for NHI 2

NHI 2 is an LDHA inhibitor; reduces lactate production in HeLa cells. Inhibits cell growth of LPC006 and PANC-1 cells in vitro. Enhances the antiproliferative effects of Gemcitabine (Cat. No. 3259) on LPC006 and PANC-1 cells. Also inhibits ferroptosis.

Compound Libraries for NHI 2

NHI 2 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for NHI 2

M. Wt	335.28
Formula	C₁₇H₁₂F₃NO₃
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1269802-97-2
PubChem ID	51355147
InChi Key	YPPFWRWCZNXINO-UHFFFAOYSA-N
Smiles	ON2C1=CC(C3=CC=CC=C3)=CC(C(F)(F)F)=C1C=C2C(OC)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NHI 2

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	33.53	100
	ethanol	33.53	100

Preparing Stock Solutions for NHI 2

The following data is based on the product molecular weight 335.28.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.98 mL	14.91 mL	29.83 mL
5 mM	0.60 mL	2.98 mL	5.97 mL
10 mM	0.30 mL	1.49 mL	2.98 mL
50 mM	0.06 mL	0.30 mL	0.60 mL

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Reconstitution Calculator

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References for NHI 2

References are publications that support the biological activity of the product.

Jacobsen APD-containing cyclolipodepsipeptides target mitochondrial function in hypoxic cancer cells. Cell Chem.Biol. 2018 PMID: 30122371
Calvaresi Dual targeting of the Warburg effect with a glucose-conjugated lactate dehydrogenase inhibitor. Chembiochem 2013 PMID: 24174263
Granchi Assessing the differential action on cancer cells of LDH-A inhibitors based on the N-hydroxyindole-2-carboxylate (NHI) and malonic (Mal) scaffolds. Org.Biomol.Chem. 2013 PMID: 23986182
Maftouh Synergistic interaction of novel lactate dehydrogenase inhibitors with gemcit. against pancreatic cancer cells in hypoxia. Br.J.Cancer 2014 PMID: 24178759