NF 110

Tocris Bioscience, part of Bio-Techne | Catalog # 2548

COA / SDS

Potent P2X3 antagonist

Key Product Details

Description:	Potent P2X₃ antagonist
Chemical Name:	4,4',4'',4'''-[Carbonylbis[imino-5,1,3-benzenetriylbis(carbonylimino)]]tetrakisbenzenesulfonic acid tetrasodium salt
Purity:	≥90% (HPLC)
Molecular Weight:	1096.9

View Full Technical Details for NF 110

Technical Data
Citations

Biological Activity for NF 110

NF 110 is a high affinity P2X₃ receptor antagonist (K_i values are 36, 82 and 4144 nM for P2X₃, P2X₁ and P2X₂ recombinant receptors respectively). Shows no activity at P2Y₁, P2Y₂ and P2Y₁₁ receptors (IC₅₀ > 10 μM). Potently inhibits α,β-meATP-evoked desensitizing currents in rat DRG neurons (IC₅₀ = 527 nM). Shows antitumor activity against several tumor types. Also inhibits DNA-binding activity of HMGA2 (IC₅₀ = 0.87 μM).

This product is supplied with a high degree of hydration and some residual NaCl, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

Technical Data for NF 110

M. Wt	1096.9
Formula	C₄₁H₂₈N₆Na₄O₁₇S₄
Storage	Desiccate at RT
Purity	≥90% (HPLC)
CAS Number	111150-22-2
PubChem ID	16066783
InChi Key	AQJHZNCSXLBXMY-UHFFFAOYSA-J
Smiles	[Na+].[Na+].[Na+].[Na+].[O-]S(=O)(=O)C1=CC=C(NC(=O)C2=CC(=CC(NC(=O)NC3=CC(=CC(=C3)C(=O)NC3=CC=C(C=C3)S([O-])(=O)=O)C(=O)NC3=CC=C(C=C3)S([O-])(=O)=O)=C2)C(=O)NC2=CC=C(C=C2)S([O-])(=O)=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for NF 110

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	40

Preparing Stock Solutions for NF 110

The following data is based on the product molecular weight 1096.9.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.37 mM	2.46 mL	12.32 mL	24.64 mL
1.85 mM	0.49 mL	2.46 mL	4.93 mL
3.7 mM	0.25 mL	1.23 mL	2.46 mL
18.5 mM	0.05 mL	0.25 mL	0.49 mL

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Reconstitution Calculator

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References for NF 110

References are publications that support the biological activity of the product.

Su Identification of HMGA2 inhibitors by AlphaScreen-based ultra-high-throughput screening assays. Sci.Rep. 2020 PMID: 33139812
Kassack Structure-activity relationships of analogues of NF449 confirm NF449 as the most potent and selective known P2X₁ receptor antagonist. Eur.J.Med.Chem. 2004 PMID: 15072843
Hausmann The suramin analog 4,4',4'',4'''-(Carbonylbis(imino-5,1,3-benzenetriylbis (carbonylimino)))tetra-kis-benzenesulfonic acid (NF110) potently blocks P2X3 receptors: subtype selectivity is determined by location of sulfonic Mol.Pharmacol. 2006 PMID: 16551782
Dhar Antitumour activity of suramin analogues in human tumour cell lines and primary cultures of tumour cells from patients. Eur.J.Cancer 2000 PMID: 10762755