Necrostatin-1

Name: Necrostatin-1
Brand: Tocris Bioscience
SKU: 2324
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 2324

(2)

Citations (67)

COA / SDS

RIP1 kinase inhibitor; inhibits necroptosis

Key Product Details

Description:	RIP1 kinase inhibitor; inhibits necroptosis
Chemical Name:	5-(1H-Indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone
Purity:	≥98% (HPLC)
Molecular Weight:	259.33

View Full Technical Details for Necrostatin-1

Technical Data
Citations / Reviews (2)

Biological Activity for Necrostatin-1

Necrostatin-1 is an ATP-competitive, allosteric inhibitor of receptor-interacting protein kinase 1 (RIPK1) (EC₅₀ = 182 nM). Blocks non-apoptotic cell death via inhibition of a specific cellular pathway, necroptosis, which leads to necrosis (EC₅₀ = 494 nM). Reduces ischemic brain injury in a mouse model of stroke.

Compound Libraries for Necrostatin-1

Necrostatin-1 is also offered as part of the Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for Necrostatin-1

M. Wt	259.33
Formula	C₁₃H₁₃N₃OS
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	4311-88-0
PubChem ID	2828334
InChi Key	TXUWMXQFNYDOEZ-UHFFFAOYSA-N
Smiles	CN1C(=S)NC(CC2=CNC3=CC=CC=C23)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Necrostatin-1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	7.78	30

Preparing Stock Solutions for Necrostatin-1

The following data is based on the product molecular weight 259.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.3 mM	12.85 mL	64.27 mL	128.54 mL
1.5 mM	2.57 mL	12.85 mL	25.71 mL
3 mM	1.29 mL	6.43 mL	12.85 mL
15 mM	0.26 mL	1.29 mL	2.57 mL

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References for Necrostatin-1

References are publications that support the biological activity of the product.

Degterev Identification of RIP1 kinase as a specific cellular target of necroSTAT. Nat.Chem.Biol. 2008 PMID: 18408713
Degterev Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. Nat.Chem.Biol. 2005 PMID: 16408008