MPD 2
Tocris Bioscience, part of Bio-Techne | Catalog # 8802
SARS-CoV-2 Mpro Degrader (PROTAC®)
Product Description
MPD 2 is a SARS-CoV-2 Mpro Degrader (PROTAC®) (DC50 = 296 nM in 293T cells). Mpro degradation is time-dependent and CRBN-mediated. Antiviral in A549-ACE2 cells infected with several SARS-CoV-2 strains (EC50 = 492 nM at delta variant) and against a nirmatrelvir-resistant mutant of SARS-CoV-2, NSP5 E166A (EC50 = 460 nM).PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.
Scientific Data Images for MPD 2
Application of MPD 2.
Western blot data showing dose responsive degradation with MPD 2 on transiently expressed SARS-CoV-2 Main Protease in HEK293 cells. 1st Ab: HRP Anti-V5 tag antibody [E10/V4RR] (ab173837) (1:2000), 2nd Ab: Anti-Mouse HRP (1:5000). Loading control: 1st Ab: Anti-alpha - Tubulin antibody [DM1A] - (1:10000) 2nd Ab: Anti-mouse HRP (1:5000). Data provided by Brönstrup Lab at the Helmholtz Centre for Infection Research.Product Specifications
Molecular Weight
973.13
Formula
C51H68N6O13
Storage
Store at -20°C
Chemical Name
3-((6-((2-(2,6-Dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)hexyl)oxy)benzyl ((2S,3R)-3-(tert-butoxy)-1-(((S)-3-cyclohexyl-1-oxo-1-(((S)-1-oxo-3-((S)-2-oxopyrrolidin-3-yl)propan-2-yl)amino)propan-2-yl)amino)-1-oxobutan-2-yl)carbamate
PubChem ID
171663340
InChI Key
ZKSDAFLRLHGTNH-DTKLLKMQSA-N
SMILES
O=C1[C@@H](CCN1)C[C@H](NC([C@@H](NC([C@@H](NC(OCC2=CC(OCCCCCCOC3=C4C(C(N(C4=O)C5CCC(NC5=O)=O)=O)=CC=C3)=CC=C2)=O)[C@@H](C)OC(C)(C)C)=O)CC6CCCCC6)=O)C([H])=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 97.31 | 100 |
Preparing Stock Solutions
for MPD 2
The following data is based on the product molecular weight 973.13
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.03 mL | 5.14 mL | 10.28 mL |
| 5 mM | 0.21 mL | 1.03 mL | 2.06 mL |
| 10 mM | 0.10 mL | 0.51 mL | 1.03 mL |
| 50 mM | 0.02 mL | 0.10 mL | 0.21 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Alugubelli Discovery of first-in-class PROTAC Degraders of SARS-CoV-2 main protease. J.Med.Chem. 2024 PMID: 38608245
Product Specific Notices for MPD 2
For research use only
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