MK 2206 dihydrochloride

Name: MK 2206 dihydrochloride
Brand: Tocris Bioscience
SKU: 7850

Tocris Bioscience, part of Bio-Techne | Catalog # 7850

COA / SDS

Potent and selective allosteric Akt1, Akt2 and Akt3 inhibitor

Key Product Details

Description:	Potent and selective allosteric Akt1, Akt2 and Akt3 inhibitor
Chemical Name:	8-[4-(1-Aminocyclobutyl)phenyl]-9-phenyl-1,2,4-triazolo[3,4-f][1,6]naphthyridin-3(2H)-one dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	480.39

View Full Technical Details for MK 2206 dihydrochloride

Technical Data
Citations

Biological Activity for MK 2206 dihydrochloride

MK 2206 dihydrochloride is a potent and selective allosteric Akt inhibitor (IC₅₀ values are 5 nM, 12 nM, and 65 nM for Akt1, Akt2, and Akt3, respectively). MK 2206 requires the Pleckstrin homology domain for its activity, and exhibits no inhibitory activity in a panel of 250 tested protein kinases. MK 2206 induces growth inhibition of different cancer cell lines (IC₅₀ in the range 3.4 and 28.6 μmol/L) and enhances antitumor efficacy of a range of standard chemotherapeutics. It synergistically inhibits cell proliferation of human lung and breast cancer cells in combination with Erlotinib (Cat. No. 7194) by suppressing both the Ras/Erk and PI 3-K pathways in vitro and in vivo. MK 2206 can be used to block the IL-22 - Akt signaling pathway for studying paneth cell differentiation.

Technical Data for MK 2206 dihydrochloride

M. Wt	480.39
Formula	C₂₅H₂₁N₅O.2HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1032350-13-2
PubChem ID	46930998
InChi Key	HWUHTJIKQZZBRA-UHFFFAOYSA-N
Smiles	O=C1NN=C2C3=C(C=CN12)N=C(C4=CC=C(C5(CCC5)N)C=C4)C(C6=CC=CC=C6)=C3.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for MK 2206 dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	24.02	50

Preparing Stock Solutions for MK 2206 dihydrochloride

The following data is based on the product molecular weight 480.39.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.16 mL	20.82 mL	41.63 mL
2.5 mM	0.83 mL	4.16 mL	8.33 mL
5 mM	0.42 mL	2.08 mL	4.16 mL
25 mM	0.08 mL	0.42 mL	0.83 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for MK 2206 dihydrochloride

References are publications that support the biological activity of the product.

Hirai MK-2206, an allosteric Akt Inhibitor, enhances antitumor efficacy by standard chemotherapeutic agents or molecular targeted drugs in vitro and in vivo. Mol.Cancer Ther. 2010 PMID: 20571069
Li Abstract #DDT01-1: MK-2206: A potent oral allosteric AKT inhibitor. AACR Annual Meeting
He Optimized human intestinal organoid model reveals interleukin-22-dependency of paneth cell formation. Cell Stem Cell 2022 PMID: 36002022