MitoBoronic acid

Name: MitoBoronic acid
Brand: Tocris Bioscience
SKU: 7949

Tocris Bioscience, part of Bio-Techne | Catalog # 7949

COA / SDS

Ratiometric mass spectrometry mitochondrial hydrogen peroxide (H2O2) probe

Key Product Details

Description:	Ratiometric mass spectrometry mitochondrial hydrogen peroxide (H₂O₂) probe
Chemical Name:	[(3-Boronophenyl)methyl]triphenylphosphonium bromide
Purity:	≥98% (HPLC)
Molecular Weight:	477.14

View Full Technical Details for MitoBoronic acid

Technical Data
Citations

Biological Activity for MitoBoronic acid

MitoBoronic acid (MitoB) is a ratiometric mass spectrometry mitochondrial hydrogen peroxide (H₂O₂) probe. MitoBoronic acid is taken up into cells and accumulates inside the mitochondrial matrix where it reacts to H₂O₂ to be converted in MitoP. Measurement of MitoB/MitoP ratio by liquid chromatography tandem mass spectrometry (LC-MS/MS) is an indicator of the mitochondrial matrix H₂O₂ concentration. This method can be used in vitro and in vivo.

Technical Data for MitoBoronic acid

M. Wt	477.14
Formula	C₂₅H₂₃BBrO₂P
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1247025-84-8
PubChem ID	91885451
InChi Key	VDNRROALFUZCBO-UHFFFAOYSA-M
Smiles	OB(C1=CC=CC(C[P+](C2=CC=CC=C2)(C3=CC=CC=C3)C4=CC=CC=C4)=C1)O.[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for MitoBoronic acid

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	47.71	100
	ethanol	23.86	50 with gentle warming

Preparing Stock Solutions for MitoBoronic acid

The following data is based on the product molecular weight 477.14.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.10 mL	10.48 mL	20.96 mL
5 mM	0.42 mL	2.10 mL	4.19 mL
10 mM	0.21 mL	1.05 mL	2.10 mL
50 mM	0.04 mL	0.21 mL	0.42 mL

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Reconstitution Calculator

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References for MitoBoronic acid

References are publications that support the biological activity of the product.

Gruszczyk Mitochondrial metabolism and bioenergetic function in an anoxic isolated adult mouse cardiomyocyte model of in vivo cardiac ischemia-reperfusion injury. Redox.Biol. 2022 PMID: 35749842
Cochemé Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix. Cell Metab. 2011 PMID: 21356523
Cochemé Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila. Nat.Protoc. 2012 PMID: 22517261