L-Ascorbic acid

Name: L -Ascorbic acid
Brand: Tocris Bioscience
SKU: 4055
Rating: 5 (2 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4055

(2)

Citations (64)

COA / SDS

Enhances the generation of iPSCs; increases reprogramming efficiency

Key Product Details

Description:	Enhances the generation of iPSCs; increases reprogramming efficiency
Alternative Names:	L-Ascorbate,Vitamin C
Chemical Name:	3-Oxo-L-gulofuranolactone
Purity:	≥99% (HPLC)
Molecular Weight:	176.12

View Full Technical Details for L-Ascorbic acid

Technical Data
Citations / Reviews (2)

Biological Activity for L-Ascorbic acid

L-Ascorbic acid is an inhibitor of Ca_v3.2 channels (IC₅₀ = 6.5 μM); displays no effect on Ca_v3.1 or Ca_v3.3 channels heterologously expressed in HEK 293 cells. Also enhances the generation of induced pluripotent stem cells (iPSCs) from mouse and human somatic cells by increasing reprogramming efficiency. Commonly used antifade reagent in live cell microscopy. Used for the generation of midbrain dopaminergic neurons from hPSCs or CiPSCs from MEFs (see protocols below). Naturally occurring antioxidant.

L-Ascorbic acid synthesized to Ancillary Material Grade also available.

For more information about how L-Ascorbic acid may be used, see our protocol: Highly Efficient Generation of CiPSCs from MEFs, Generating Midbrain Dopaminergic Neurons from hPSCs.

Technical Data for L-Ascorbic acid

M. Wt	176.12
Formula	C₆H₈O₆
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	50-81-7
PubChem ID	54670067
InChi Key	CIWBSHSKHKDKBQ-JLAZNSOCSA-N
Smiles	OC1=C(O)[C@]([C@@H](O)CO)([H])OC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for L-Ascorbic acid

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	88.06	500

Preparing Stock Solutions for L-Ascorbic acid

The following data is based on the product molecular weight 176.12.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
5 mM	1.14 mL	5.68 mL	11.36 mL
25 mM	0.23 mL	1.14 mL	2.27 mL
50 mM	0.11 mL	0.57 mL	1.14 mL
250 mM	0.02 mL	0.11 mL	0.23 mL

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References for L-Ascorbic acid

References are publications that support the biological activity of the product.

Cordes Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy. Phys.Chem.Chem.Phys. 2011 PMID: 21311807
Nelson Molecular mechanisms of subtype-specific inhibition of neuronal T-type calcium channels by ascorbate. J.Neurosci. 2007 PMID: 18003836
Esteban Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell 2010 PMID: 20036631
Padayatty Vitamin C as an antioxidant: evaluation of its role in disease prevention. J.Am.Coll.Nutr. 2003 PMID: 12569111