K 22

Name: K 22
Brand: Tocris Bioscience
SKU: 7338

Tocris Bioscience, part of Bio-Techne | Catalog # 7338

COA / SDS

Inhibits coronavirus RNA replication; impairs double membrane vesicle (DMV) formation

Key Product Details

Description:	Inhibits coronavirus RNA replication; impairs double membrane vesicle (DMV) formation
Chemical Name:	N-[(1Z)-1-[[4-(4-Bromophenyl)-4-hydroxy-1-piperidinyl]carbonyl]-2-phenylethenyl]benzamide
Purity:	≥98% (HPLC)
Molecular Weight:	505.41

View Full Technical Details for K 22

Technical Data
Citations

Biological Activity for K 22

K 22 is a viral RNA synthesis inhibitor; targets membrane-bound coronaviral RNA replication by inhibiting double membrane vesicle (DMV) formation. Inhibits HCoV-229E infection of MRC-5 cells in a plaque forming assay (IC₅₀ = 0.7 μM). Also inhibits HCoV-229E and MERS-CoV replication in human airway epithelia cultures.

Technical Data for K 22

M. Wt	505.41
Formula	C₂₇H₂₅BrN₂O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2141978-86-9
PubChem ID	5433970
InChi Key	CMBUSPXASRNJHI-CLCOLTQESA-N
Smiles	OC1(C2=CC=C(Br)C=C2)CCN(C(/C(=C/C3=CC=CC=C3)/NC(=O)C4=CC=CC=C4)=O)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for K 22

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	50.54	100

Preparing Stock Solutions for K 22

The following data is based on the product molecular weight 505.41.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.98 mL	9.89 mL	19.79 mL
5 mM	0.40 mL	1.98 mL	3.96 mL
10 mM	0.20 mL	0.99 mL	1.98 mL
50 mM	0.04 mL	0.20 mL	0.40 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for K 22

References are publications that support the biological activity of the product.

Lundin Targeting membrane-bound viral RNA synthesis reveals potent inhibition of diverse coronaviruses including the middle East respiratory syndrome virus. PLoS Pathog. 2014 PMID: 24874215