JW 74

Name: JW 74
Brand: Tocris Bioscience
SKU: 5653

Tocris Bioscience, part of Bio-Techne | Catalog # 5653

Citations (9)

COA / SDS

Wnt pathway inhibitor

Key Product Details

Description:	Wnt pathway inhibitor
Chemical Name:	4-[4-(4-Methoxyphenyl)-5-[[[3-(4-methylphenyl)-1,2,4-oxadiazol-5-yl]methyl]thio]-4H-1,2,4-triazol-3-yl]-pyridine
Purity:	≥98% (HPLC)
Molecular Weight:	456.52

View Full Technical Details for JW 74

Technical Data
Citations

Biological Activity for JW 74

JW 74 is an inhibitor of canonical Wnt signaling (IC₅₀ = 790 nM). Targets the β-catenin destruction complex (GSK-3 beta /AXIN/APC). Increases AXIN2 and decreases active β-catenin levels in colorectal cancer (CRC) cells. Also arrests cell cycle in G₁ and inhibits proliferation of CRC cells in vitro. Inhibits tumor growth and formation in mouse models.

Compound Libraries for JW 74

JW 74 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for JW 74

M. Wt	456.52
Formula	C₂₄H₂₀N₆O₂S
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	863405-60-1
PubChem ID	16013152
InChi Key	KRIKILRRJCIWNB-UHFFFAOYSA-N
Smiles	COC1=CC=C(N2C(SCC3=NC(C4=CC=C(C)C=C4)=NO3)=NN=C2C5=CC=NC=C5)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for JW 74

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	45.65	100

Preparing Stock Solutions for JW 74

The following data is based on the product molecular weight 456.52.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.19 mL	10.95 mL	21.90 mL
5 mM	0.44 mL	2.19 mL	4.38 mL
10 mM	0.22 mL	1.10 mL	2.19 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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References for JW 74

References are publications that support the biological activity of the product.

Waaler Novel synthetic antagonists of canonical Wnt signaling inhibit colorectal cancer cell growth. Cancer Res. 2011 PMID: 21199802