IWP 4

Name: IWP 4
Brand: Tocris Bioscience
SKU: 5214
Rating: 4 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 5214

Citations (61)

COA / SDS

Potent inhibitor of Wnt/β-catenin signaling

Key Product Details

Description:	Potent inhibitor of Wnt/β-catenin signaling
Chemical Name:	N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-3-(2-methoxyphenyl)-4-oxothieno[3,2-d]pyrimidin-2-yl)thio]-acetamide
Purity:	≥98% (HPLC)
Molecular Weight:	496.62

View Full Technical Details for IWP 4

Technical Data
Citations

Biological Activity for IWP 4

IWP 4 is a potent inhibitor of Wnt/β-catenin signaling (IC₅₀ = 25 nM). Has minimal effect on Notch and Hedgehog signaling pathways. Induces differentiation of cardiomyocytes from human ESCs and iPSCs.

Technical Data for IWP 4

M. Wt	496.62
Formula	C₂₃H₂₀N₄O₃S₃
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	686772-17-8
PubChem ID	2155264
InChi Key	RHUJMHOIQBDFQR-UHFFFAOYSA-N
Smiles	O=C1C3=C(CCS3)N=C(SCC(NC4=NC(C=CC(C)=C5)=C5S4)=O)N1C2=C(OC)C=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for IWP 4

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	0.5	1

Preparing Stock Solutions for IWP 4

The following data is based on the product molecular weight 496.62.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.01 mM	201.36 mL	1,006.81 mL	2,013.61 mL
0.05 mM	40.27 mL	201.36 mL	402.72 mL
0.1 mM	20.14 mL	100.68 mL	201.36 mL
0.5 mM	4.03 mL	20.14 mL	40.27 mL

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Reconstitution Calculator

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References for IWP 4

References are publications that support the biological activity of the product.

Noor 3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts. Adv Sci (Weinh) 2019 PMID: 31179230
Hoang Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells. Nat Protoc. 2018 PMID: 29543795
Chen Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat.Chem.Biol. 2009 PMID: 19125156
Narytnyk Differentiation of human epidermal neural crest stem cells (hEPI-NCSC) into virtually homogenous populations of DArgic neurons. Stem Cell Rev. 2014 PMID: 24399192
Lian Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc.Natl.Acad.Sci.U.S.A. 2012 PMID: 22645348