ISX 9

Product Description

ISX 9 is a neurogenic agent. Mediates neuroD reporter gene induction via activation of Ca²⁺ influx; increases expression of neurogenic differentiation 1 (NeuroD1) transcription factor. Induces neuronal differentiation in human cortical neuronal cells (HCN), adult mouse whole brain (MWB) and subventricular zone (SVZ) progenitors. ISX 9 is also shown to induce cardiomyogenic differentiation of Notch-activated epicardium-derived cells in vitro. When used in combination with other small molecules, ISX 9 directs differentiation of pancreatic progenitors towards β cells. Used in protocol to differentiate fibroblasts directly into neurons (see below).

ISX 9 synthesized to Ancillary Material Grade also available.

For more information about how ISX 9 may be used, see our protocol: Transdifferentiating Fibroblasts into Neurons.

Solubility

Preparing Stock Solutions for ISX 9

The following data is based on the product molecular weight 234.27

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.27 mL	21.34 mL	42.69 mL
5 mM	0.85 mL	4.27 mL	8.54 mL
10 mM	0.43 mL	2.13 mL	4.27 mL
50 mM	0.09 mL	0.43 mL	0.85 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Liu Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells. Nat.Commun. 2021 PMID: 34099664
• Russell Targeting native adult heart progenitors with cardiogenic small molecules. ACS Chem.Biol. 2012 PMID: 22413910
• Dioum A small molecule differentiation inducer increases Ins production by pancreatic β cells. Proc.Natl.Acad.Sci.U.S.A. 2011 PMID: 22143803
• Zhang Small-molecule blocks malignant astrocyte proliferation and induces neuronal gene expression. Differentiation 2011 PMID: 21419563
• Schneider Small-molecule activation of neuronal cell fate. Nat.Chem.Biol 2008 PMID: 18552832