Hygromycin B
Tocris Bioscience, part of Bio-Techne | Catalog # 4137
Aminoglycoside antibiotic; active against prokaryotic and eukaryotic cells
Key Product Details
Description
Aminoglycoside antibiotic; active against prokaryotic and eukaryotic cells
Product Description
Hygromycin B is an aminoglycoside antibiotic. Active against both prokaryotic and eukaryotic cells. Inhibits protein synthesis by binding to the 30S subunit and inhibiting the ribosomal translocation step of elongation.Product Specifications
Molecular Weight
527.52
Formula
C20H37N3O13
Storage
Store at +4°C
Purity
≥90% (HPLC)
Chemical Name
O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
CAS Number
31282-04-9
PubChem ID
90488914
InChI Key
GRRNUXAQVGOGFE-SZNRJEGWSA-N
SMILES
O[C@H]([C@@H](NC)C[C@@H](N)[C@@H]4O)C4O[C@H]1[C@]2([H])[C@](OC3(O[C@]([H])([C@@H](N)CO)[C@H](O)[C@@H](O)[C@H]3O)O2)([H])[C@@H](O)[C@@H](CO)O1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 50 |
Preparing Stock Solutions
for Hygromycin B
The following data is based on the product molecular weight 527.52
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.95 mM | 2.00 mL | 9.98 mL | 19.95 mL |
| 4.75 mM | 0.40 mL | 2.00 mL | 3.99 mL |
| 9.5 mM | 0.20 mL | 1.00 mL | 2.00 mL |
| 47.5 mM | 0.04 mL | 0.20 mL | 0.40 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Brodersen The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell 2000 PMID: 11163189
- Ganoza and Kiel A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes. Antimicrob.Agents Chemother. 2001 PMID: 11557474
- Blochlinger and Diggelmann Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eukaryotic cells. Mol.Cell.Biol. 1984 PMID: 6098829
Product Specific Notices for Hygromycin B
For research use only
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