H 89 dihydrochloride is a protein kinase A inhibitor that also inhibits several other kinases (IC₅₀ values are 80, 120, 135, 270, 2600 and 2800 nM for S6K1, MSK1, PKA, ROCKII, PKBα and MAPKAP-K1b). Exhibits antinociceptive activity. Enhances survival and clonogenicity of dissociated human ESCs through ROCK inhibition.

Product Specifications

Molecular Weight

519.28

Formula

C₂₀H₂₀BrN₃O₂S.2HCl

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

N-[2-[[3-(4-Bromophenyl)-2-propenyl]amino]ethyl]-5-isoquinolinesulfonamide dihydrochloride

CAS Number

130964-39-5

PubChem ID

5702541

InChI Key

GELOGQJVGPIKAM-WTVBWJGASA-N

SMILES

O=S(C2=CC=CC1=CN=CC=C12)(NCCNC/C=C/C3=CC=C(Br)C=C3)=O.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	12.98	25
	DMSO	51.93	100

Preparing Stock Solutions for H 89 dihydrochloride

The following data is based on the product molecular weight 519.28

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.93 mL	9.63 mL	19.26 mL
5 mM	0.39 mL	1.93 mL	3.85 mL
10 mM	0.19 mL	0.96 mL	1.93 mL
50 mM	0.04 mL	0.19 mL	0.39 mL

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Background References

References are publications that support the biological activity of the product.

Zhang Protein kinase A inhibitor, H89, enhances survival and clonogenicity of dissociated human embryonic stem cells through Rho-associated coiled-coil containing protein kinase (ROCK) inhibition. Hum.Reprod. 2016 PMID: 26848187
Vitolo Amyloid β-peptide inhibition of the PKA/CREB pathway and long-term potentiation: reversibility by drugs that enhance cAMP signaling. Proct.Natl.Acad.Sci.USA 2002 PMID: 12244210
Dolan and Nolan Biphasic modulation of nociceptive processing by the cyclic AMP-protein kinase A signalling pathway in sheep spinal cord. Neurosci.Lett. 2001 PMID: 11514065
Lochner and Moolman The many faces of H89: a review. Cardiovasc.Drug Rev. 2006 PMID: 17214602

H 89 dihydrochloride

Tocris Bioscience, part of Bio-Techne | Catalog # 2910

Key Product Details

Description

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Product Description