H-7 dihydrochloride

Name: H-7 dihydrochloride
Brand: Tocris Bioscience
SKU: 0542
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 0542

(1)

Citations (6)

COA / SDS

Protein kinase inhibitor

Key Product Details

Description:	Protein kinase inhibitor
Chemical Name:	(±)-1-(5-Isoquinolinesulphonyl)-2-methylpiperazine dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	364.29

View Full Technical Details for H-7 dihydrochloride

Technical Data
Citations / Reviews (1)

Biological Activity for H-7 dihydrochloride

H-7 dihydrochloride is a protein kinase inhibitor (IC₅₀ values for inhibition of PKA, PKG, PKC and myosin light chain kinase are 3.0, 5.8, 6.0 and 97.0 μM, respectively. H-7 shows antiviral activity against influenza A (IC₅₀ = 10 μM) in vitro. H-7 also inhibits interleukin-stimulated secretion of IgM and blocks PMA-stimulated interleukin 1β production.

Compound Libraries for H-7 dihydrochloride

H-7 dihydrochloride is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for H-7 dihydrochloride

M. Wt	364.29
Formula	C₁₄H₁₇N₃O₂S.2HCl
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	108930-17-2
PubChem ID	73332
InChi Key	OARGPFMFRLLKPF-UHFFFAOYSA-N
Smiles	Cl.Cl.CC1CNCCN1S(=O)(=O)C1=CC=CC2=CN=CC=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for H-7 dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	36.43	100
	DMSO	7.29	20

Preparing Stock Solutions for H-7 dihydrochloride

The following data is based on the product molecular weight 364.29.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.75 mL	13.73 mL	27.45 mL
5 mM	0.55 mL	2.75 mL	5.49 mL
10 mM	0.27 mL	1.37 mL	2.75 mL
50 mM	0.05 mL	0.27 mL	0.55 mL

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Reconstitution Calculator

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References for H-7 dihydrochloride

References are publications that support the biological activity of the product.

Hurme Control of interleukin-1 beta expression by protein kinase C and cyclic adenosine monophosphate in myeloid leukemia cells Blood 1990 PMID: 2175219
Kurokawa Inhibitory effect of protein kinase C inhibitor on the replication of influenza type A virus. J.Gen.Virol. 1990 PMID: 1698925
Quick The structure and biological activities of the widely used protein kinase inhibitor, H7, differ depending on the commercial source. Biochem.Biophys.Res.Commun. 1992 PMID: 1530632
Ratz Effect of the kinase inhibitor, H-7, on stress, crossbridge phosphorylation, muscle shortening and inositol phosphate production in rabbit arteries. J.Pharmacol.Exp.Ther. 1990 PMID: 2299593
Hidaka Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry 1984 PMID: 6238627