Fludarabine

Name: Fludarabine
Brand: Tocris Bioscience
SKU: 3495
Rating: 5 (1 reviews)

Catalog # 3495 | Tocris Bioscience a Bio-Techne Brand

(1)

Citations (10)

COA / SDS

Purine analog; inhibits DNA synthesis

Key Product Details

Description:	Purine analog; inhibits DNA synthesis
Chemical Name:	9-β-D-Arabinofuranosyl-2-fluoro-9H-purin-6-amine
Purity:	≥99% (HPLC)
Molecular Weight:	285.23

View Full Technical Details for Fludarabine

Technical Data
Citations (10) / Reviews (1)

Biological Activity for Fludarabine

Fludarabine is a purine analog that inhibits DNA synthesis. Exhibits antiproliferative activity (IC₅₀ = 1.54 μM in RPMI cells) and triggers apoptosis through increasing Bax and decreasing Bid, XIAP and survivin expression. Inhibits cytokine-induced activation of STAT1 and STAT1-dependent gene transcription in lymphocytes. Also displays anticancer activity against hematological malignancies in vivo.

Compound Libraries for Fludarabine

Fludarabine is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Antiviral Library and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Fludarabine

M. Wt	285.23
Formula	C₁₀H₁₂FN₅O₄
Storage	Desiccate at +4°C
Purity	≥99% (HPLC)
CAS Number	21679-14-1
PubChem ID	657237
InChi Key	HBUBKKRHXORPQB-FJFJXFQQSA-N
Smiles	O[C@@H]1[C@@H](CO)O[C@@H](N3C2=NC(F)=NC(N)=C2N=C3)[C@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Fludarabine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	28.52	100

Preparing Stock Solutions for Fludarabine

The following data is based on the product molecular weight 285.23.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.51 mL	17.53 mL	35.06 mL
5 mM	0.70 mL	3.51 mL	7.01 mL
10 mM	0.35 mL	1.75 mL	3.51 mL
50 mM	0.07 mL	0.35 mL	0.70 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Fludarabine

References are publications that support the biological activity of the product.

Meng Antitumor activity of fludar. against human multiple myeloma in vitro and in vivo. Eur.J.Haematol. 2007 PMID: 17976186
Torella Fludarabine prevents smooth muscle proliferation in vitro and neointimal hyperplasia in vivo through specific inhibition of STAT-1 activation. Am.J.Physiol.Heart Circ.Physiol. 2007 PMID: 17293493
Bellosillo In vitro evaluation of fludar. in combination with cyclophosph. and/or mitoxan. in B-cell chronic lymphocytic leukemia. Blood 1999 PMID: 10515887
Frank Fludarabine-induced immunosuppression is associated with inhibition of STAT1 signaling. Nat.Med. 1999 PMID: 10202937

Citations for Fludarabine (10)

Showing 1 - 10 of 10 citations Showing All

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Fludarabine include:

Yuwei et al. (2023) Age-dependent Pdgfrβ signaling drives adipocyte progenitor dysfunction to alter the beige adipogenic niche in male mice. Nat Commun. 14:1806
PMID: 37002214
Xiang et al. (2022) A positive feedback loop between gastric cancer cells and tumor-associated macrophage induces malignancy progression. J Exp Clin Cancer Res. 41:174
PMID: 35562774
Alexey et al. (2022) Attenuation of SARS-CoV-2 replication and associated inflammation by concomitant targeting of viral and host cap 2'-O-ribose methyltransferases. EMBO J. 41:e111608
PMID: 35833542
Brij B et al. (2021) Resolving macrophage polarization through distinct Ca2+ entry channel that maintains intracellular signaling and mitochondrial bioenergetics. iScience. 24:103339
PMID: 34816101
Fred et al. (2020) STAT Signaling Modifies Ascl1 Chromatin Binding and Limits Neural Regeneration from Muller Glia in Adult Mouse Retina. Cell Rep. 30:2195-2208.e5
PMID: 32075759
Beate et al. (2020) Keratinocytes costimulate naive human T cells via CD2: a potential target to prevent the development of proinflammatory Th1 cells in the skin. Cell Mol Immunol. 17:380-394
PMID: 31324882
Haiyan et al. (2019) E3-ubiquitin ligase TRIM6 aggravates myocardial ischemia/reperfusion injury via promoting STAT1-dependent cardiomyocyte apoptosis. Aging (Albany NY). 11:3536-3550
PMID: 31171760
Odendall et al. (2014) Diverse intracellular pathogens activate type III IF. expression from peroxisomes. Nat Immunol. 15:717
PMID: 24952503
Nathalie et al. (2014) Fludarabine downregulates indoleamine 2,3-dioxygenase in tumors via a proteasome-mediated degradation mechanism. PLoS One. 9:e99211
PMID: 24911872
Robinson et al. (2013) RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs. PLoS One. 8:e78641
PMID: 24265703

There are no citations that match your criteria.

Showing 1 - 10 of 10 citations Showing All

Customer Reviews for Fludarabine (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

STAT1 inhibitor in IFN beta signaling

Name: Richa Rani

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: Hepatic stellate cells and hepatocytes

I used it to check its effect on drug stimulated hepatic stellate cell and its signaling.

There are no reviews that match your criteria.