FerroOrange New
Tocris Bioscience, part of Bio-Techne | Catalog # 8846
Sensitive and cell-permeable fluorogenic probe for the detection of labile Fe(II) in live cells; suitable for high-throughput assays
Key Product Details
Description
Sensitive and cell-permeable fluorogenic probe for the detection of labile Fe(II) in live cells; suitable for high-throughput assays
Alternative Names
RhoNox-4
Product Description
Key information: FerroOrange (RhoNox-4) is a highly sensitive and robust fluorogenic probe for the detection of intracellular labile Fe(II). FerroOrange displays weak absorption and low fluorescence in the visible region, but becomes strongly fluorescent upon reaction with Fe(II).Used for: detection of intracellular labile Fe(II).
Application: high-throughput assays for high content imaging of subcellular labile Fe(II) in live cells; fluorescent microscopy; studies on iron homeostasis and ferroptosis.
Properties and Photophysical Data: FerroOrange has excellent cell-membrane permeability and low cell toxicity. FerroOrange has no chelating ability. For the fluorescent form, excitation and emission maxima (λ) measured in 50 mM HEPES buffer (pH 7.4, 0.2% DMSO) are 545 nm and 575 nm, respectively; extinction coefficient ε = 73,500 M-1cm-1; quantum yield φ = 0.19.
Please refer to this protocol for further information on how to use this product.
Scientific Data Images for FerroOrange
Application of FerroOrange in HeLa cells.
HeLa cells treated with chelator of iron 2,2′-bipyridyl (Bpy) (100 μmol/L) or Ammonium iron (II) sulfate (100 μmol/L) were prepared. A: Control; B: Ammonium iron (II) sulfate treated; C: Ammonium iron (II) sulfate and 2,2′-Bipyridyl (Bpy) treated. The change of intracellular Fe2+in HeLa cells was detected by FerroOrange. Excitation/Emision maxima ( lambda) = 561 nm/570-620 nm. Scale bars = 20 μm.
Application of FerroOrange in Hela cells.
Hela treated with 2 μL of 1 mmol/L FerroOrange and 2 μL of 10 mmol/L from each metal were added to 1 mL of 50 mmol/L HEPES Buffer (pH7.4). The fluorescence intensity was measured after the reaction, for 1hr, at room temperature.Optical Data
| Emission Color | Orange |
| λabs | 545 nm |
| λem | 575 nm |
| Extinction Coefficient (ε) | 73500 M-1cm-1 |
| Quantum Yield (φ) | 0.19 |
| Cell Permeable | Yes |
| Application | High-throughput assays for the detection of intracellular labile Fe(II) |
Product Specifications
Molecular Weight
684.78
Formula
C38H44N4O8
Storage
Store at +4°C
Purity
≥90% (HPLC)
Chemical Name
4-(tert-Butoxycarbonyl)-1-(3'-(4-(tert-butoxycarbonyl)piperazin-1-yl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-6'-yl)piperazine 1-oxide
CAS Number
2711762-70-6
PubChem ID
172913725
InChI Key
BPPNJBCDCLJSAA-UHFFFAOYSA-N
SMILES
O=C1OC2(C3=C1C=CC=C3)C4=CC=C(N5CCN(CC5)C(OC(C)(C)C)=O)C=C4OC6=CC([N+]7(CCN(CC7)C(OC(C)(C)C)=O)[O-])=CC=C62
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Hirayama High-throughput screening for the discovery of iron homeostasis modulators using an extremely sensitive fluorescent probe. Sensors 2020 PMID: 32885952
Product Specific Notices for FerroOrange
For research use only
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