Fasudil hydrochloride

Name: Fasudil hydrochloride
Brand: Tocris Bioscience
SKU: 0541

Tocris Bioscience, part of Bio-Techne | Catalog # 0541

Citations (41)

COA / SDS

Inhibitor of cyclic nucleotide dependent- and Rho-kinases

Key Product Details

Description:	Inhibitor of cyclic nucleotide dependent- and Rho-kinases
Alternative Names:	HA 1077
Purity:	≥98% (HPLC)
Molecular Weight:	327.83

View Full Technical Details for Fasudil hydrochloride

Technical Data
Citations

Biological Activity for Fasudil hydrochloride

Fasudil hydrochloride is a cyclic nucleotide-dependent protein kinase inhibitor and Rho-associated kinase inhibitor (IC₅₀ = 10.7 μM). Fasudil suppresses MMP-2 expression and induces apoptosis in glioblastoma cells in vivo. Fasudil is a Ca²⁺ antagonist, vasodilator and inhibits proliferation of vascular smooth muscle cells. Fasudil binds to α-synuclein to reduce aggregate formation in cellular models of Parkinson's disease, also displays neuroprotective properties and increases survival of dopaminergic neurons in vivo.

Compound Libraries for Fasudil hydrochloride

Fasudil hydrochloride is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for Fasudil hydrochloride

M. Wt	327.83
Formula	C₁₄H₁₇N₃O₂S.HCl
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	105628-07-7
PubChem ID	163751
InChi Key	LFVPBERIVUNMGV-UHFFFAOYSA-N
Smiles	Cl.O=S(=O)(N1CCCNCC1)C1=C2C=CN=CC2=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Fasudil hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	32.78	100
	DMSO	24.59	75

Preparing Stock Solutions for Fasudil hydrochloride

The following data is based on the product molecular weight 327.83.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.05 mL	15.25 mL	30.50 mL
5 mM	0.61 mL	3.05 mL	6.10 mL
10 mM	0.31 mL	1.53 mL	3.05 mL
50 mM	0.06 mL	0.31 mL	0.61 mL

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References for Fasudil hydrochloride

References are publications that support the biological activity of the product.

Pujols Chemical chaperones as novel drugs for Parkinson's Disease. Trends Mol.Med. 2020 PMID: 32277934
Deng Rho-kinase inhibitor, fasudil, suppresses glioblastoma cell line progression in vitro and in vivo. Cancer Biol Ther. 2010 PMID: 20364104
Sward Inhibition of Rho-associated kinase blocks agonist-induced Ca²⁺ sensitization of myosin phosphorylation and force in guinea-pig ileum. J.Physiol. 2000 PMID: 10618150
Satoh Neuroprotective properties of a protein kinase inhibitor against ischaemia-induced neuronal damage in rats and gerbils. Br.J.Pharmacol. 1996 PMID: 8842419
Shirotani A new type of vasodilator, HA1077, an isoquinoline derivative, inhibits proliferation of bovine vascular smooth muscle cells in culture. J.Pharmacol.Exp.Ther. 1991 PMID: 1941621
Arai Inhibition by the protein kinase inhibitor HA-1077 of the activation of NADPH oxidase in human neutrophils. Biochem.Pharmacol. 1993 PMID: 8240400