EHNA hydrochloride

Name: EHNA hydrochloride
Brand: Tocris Bioscience
SKU: 1261

Tocris Bioscience, part of Bio-Techne | Catalog # 1261

Citations (46)

COA / SDS

Adenosine deaminase inhibitor

Key Product Details

Description:	Adenosine deaminase inhibitor
Chemical Name:	erythro-9-(2-Hydroxy-3-nonyl)adenine hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	313.83

View Full Technical Details for EHNA hydrochloride

Technical Data
Citations

Biological Activity for EHNA hydrochloride

EHNA hydrochloride is a selective inhibitor of cGMP-stimulated phosphodiesterase (PDE2) (IC₅₀ = 0.8 - 4 μM). Also a potent inhibitor of adenosine deaminase. Suppresses spontaneous differentiation of human ESCs in feeder-free conditions. Also prevents directed neuronal differentiation.

Compound Libraries for EHNA hydrochloride

EHNA hydrochloride is also offered as part of the Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for EHNA hydrochloride

M. Wt	313.83
Formula	C₁₄H₂₃N₅O.HCl
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	58337-38-5
PubChem ID	11056106
InChi Key	VVDXNJRUNJMYOZ-DHXVBOOMSA-N
Smiles	NC1=NC=NC2=C1N=CN2[C@@H](CCCCCC)[C@H](O)C.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for EHNA hydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	31.38	100
	ethanol	31.38	100
	DMSO	31.38	100

Preparing Stock Solutions for EHNA hydrochloride

The following data is based on the product molecular weight 313.83.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.19 mL	15.93 mL	31.86 mL
5 mM	0.64 mL	3.19 mL	6.37 mL
10 mM	0.32 mL	1.59 mL	3.19 mL
50 mM	0.06 mL	0.32 mL	0.64 mL

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Reconstitution Calculator

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References for EHNA hydrochloride

References are publications that support the biological activity of the product.

Michie Rapid regulation of PDE-2 and PDE-4 cyclic AMP phosphodiesterase activity following ligation of the T-cell antigen receptor on thymocytes: analysis using the selective inhibitors erythro-9-(2-hydroxy-3-nonyl)-adenine (EHNA) and rolipram. Cell.Signal. 1996 PMID: 8730511
Podzuweit Isozyme selective inhibition of cGMP-stimulated cyclic nucleotide phosphodiesterases by erythro-9-(2-hydroxy-3-nonyl)adenine. Cell.Signal. 1995 PMID: 8519602
Bessodes Effect of chirality in erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) on adenosine deaminase inhibition. Biochem.Pharmacol. 1982 PMID: 7082355