DMNPE-4 AM-caged-calcium
Tocris Bioscience, part of Bio-Techne | Catalog # 5948
Calcium cage; cell permeable
Discontinued Product
5948 has been discontinued.
View all Caged Compounds products.
Product Description
DMNPE-4 AM-caged-calcium is a calcium cage. In the presence of calcium, the compound will chelate and cage calcium (Kd values are 48 and 19 nM at pH 7.2 and 7.4, respectively; Kd after uncaging = 2 mM). DMNPE-4 AM-caged-calcium is selective for Ca2+ over Mg2+ (Kd = 10 mM). Rapid and efficient calcium release occurs upon photolysis at 350 nm. It can also be used for two-photon uncaging. Extinction coefficient of 5120 M-1 cm-1, quantum yield 0.09. Cell permeable.Product Specifications
Molecular Weight
849.74
Formula
C34H47N3O22
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
Bis(acetoxymethyl) 3,12-bis(2-(acetoxymethoxy)-2-oxoethyl)-5-(4,5-dimethoxy-2-nitrophenyl)-6,9-dioxa-3,12-diazatetradecane-1,14-dioate
CAS Number
2253744-58-8
PubChem ID
137919855
InChI Key
UUZFENOXYOBVDU-UHFFFAOYSA-N
SMILES
O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)CCOCCOC(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(C=C1OC)=C(C=C1OC)[N+]([O-])=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 84.97 | 100 |
Preparing Stock Solutions
for DMNPE-4 AM-caged-calcium
The following data is based on the product molecular weight 849.74
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.18 mL | 5.88 mL | 11.77 mL |
| 5 mM | 0.24 mL | 1.18 mL | 2.35 mL |
| 10 mM | 0.12 mL | 0.59 mL | 1.18 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.24 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Gordon Brain metabolism dictates the polarity of astrocyte control over arterioles. Nature 2008 PMID: 18971930
- Ellis-Davies Tuning caged calcium: photolabile analogues of EGTA with improved optical and chelation properties. Cell Calcium 2006 PMID: 16303177
- Wang Spontaneous activity of cochlear hair cells triggered by fluid secretion mechanism in adjacent support cells Cell 2015 PMID: 26627734
Product Specific Notices for DMNPE-4 AM-caged-calcium
For research use only
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