Dihydrokainic acid

Product Description

Dihydrokainic acid is a selective EAAT2(GLT1) non-transportable inhibitor of L-glutamate and L-aspartate uptake (K_i = 23 μM). Dihydrokainic acid is 130-fold selective over EAAT1 and EAAT3 (K_i > 3 mM). In [³H]-d-Asp uptake assays in HEK293 cells expressing human EAAT2, EAAT1 and EAAT3, K_i values are 89 μM, > 3 mM and > 3 mM, respectively. Respective K_m values in a FLIPR Membrane Potential (FMP) assay, are 31 μM, > 3 mM and > 3 mM

Solubility

Preparing Stock Solutions for Dihydrokainic acid

The following data is based on the product molecular weight 215.25

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.25 mM	18.58 mL	92.92 mL	185.83 mL
1.25 mM	3.72 mL	18.58 mL	37.17 mL
2.5 mM	1.86 mL	9.29 mL	18.58 mL
12.5 mM	0.37 mL	1.86 mL	3.72 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Jensen and Bräuner-Osborne Pharmacological characterization of human excitatory amino acid transporters EAAT1, EAAT2 and EAAT3 in a fluorescence-based membrane potential assay. Biochem.Pharmacol. 2004 PMID: 15135308
• Kanal The neuronal and epithelial high affinity glutamate transporter, insights into structure and mechanism of transport. J.Biol.Chem. 1994 PMID: 7914198
• Munoz Effects of dihydrokainic acid on extracellular amino acids and neuronal excitability in the in vivo rat hippocampus. Neuropharmacology 1987 PMID: 2882438
• Arriza Functional comparisons of three glutamate transporter subtypes cloned from human motor cortex. J.Neurosci. 1994 PMID: 7521911