Dibutyryl-cAMP, sodium salt
Tocris Bioscience, part of Bio-Techne | Catalog # 1141
Cell-permeable cAMP analog
Key Product Details
Description
Cell-permeable cAMP analog
Alternative Names
Bucladesine sodium
Product Description
Dibutyryl-cAMP sodium salt is a cell-permeable analog of cAMP that activates cAMP-dependent protein kinases and is a phosphodiesterase inhibitor. Combined with other reagents, dibutyryl-cAMP promotes differentiation of neural stem/progenitor cells (NSPCs) and other cell lines (see our protocol below) and increases survival rates of NSPCs and differentiation into neurons in vivo. Promotes neurite outgrowth in cell cultures. Dibutyryl-cAMP has anti-inflammatory activity and is used to promote wound healing. Choline acetyltransferase and vesicular acetylcholine transporter mRNA are increased in cells treated with dibutyryl-cAMP.For more information about how Dibutyryl-cAMP, sodium salt may be used, see our protocol: Generating Midbrain Dopaminergic Neurons from hPSCs
Product Specifications
Molecular Weight
491.37
Formula
C18H23N5NaO8P
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
N6,O2'-Dibutyryladenosine 3',5'-cyclic monophosphate sodium salt
CAS Number
16980-89-5
PubChem ID
23663967
InChI Key
KRBZRVBLIUDQNG-JBVYASIDSA-M
SMILES
[Na+].[H][C@@]12COP([O-])(=O)O[C@@]1([H])[C@@H](OC(=O)CCC)[C@@H](O2)N1C=NC2=C1N=CN=C2NC(=O)CCC
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 49.14 | 100 | |
| DMSO | 49.14 | 100 |
Preparing Stock Solutions
for Dibutyryl-cAMP, sodium salt
The following data is based on the product molecular weight 491.37
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.04 mL | 10.18 mL | 20.35 mL |
| 5 mM | 0.41 mL | 2.04 mL | 4.07 mL |
| 10 mM | 0.20 mL | 1.02 mL | 2.04 mL |
| 50 mM | 0.04 mL | 0.20 mL | 0.41 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Kim Effects of dibutyryl cyclic-AMP on survival and neuronal differentiation of neural stem/progenitor cells transplanted into spinal cord injured rats. PLoS One 2011 PMID: 21738784
- Xia Transcriptional comparison of human induced and primary midbrain DArgic neurons. Sci.Rep. 2016 PMID: 26842779
- Hei Lack of correlation between activation of cyclic AMP-dependent protein kinase and inhibition of contraction of rat vas deferens by cyclic AMP analogs Mol.Pharmacol. 1991 PMID: 1847496
- Imamura Differential expression of dystrophin isoforms and utrophin during dibutyryl-cAMP-induced morphological differentiation of rat brain astrocytes. Proc.Natl.Acad.Sci.U.S.A. 1998 PMID: 9600931
- Carranza Protein kinase A induces recruitment of active Na+,K+-ATPase units to the plasma membrane of rat proximal convoluted tubule cells. J.Physiol. 1998 PMID: 9679177
Product Specific Notices for Dibutyryl-cAMP, sodium salt
For research use only
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