DBZ

Name: DBZ
Brand: Tocris Bioscience
SKU: 4489
Rating: 3 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4489

(1)

Citations (73)

COA / SDS

γ-secretase inhibitor; inhibits Notch pathway

Key Product Details

Description:	γ-secretase inhibitor; inhibits Notch pathway
Alternative Names:	Dibenzazepine,YO-01027
Chemical Name:	N-[(1S)-2-[[(7S)-6,7-Dihydro-5-methyl-6-oxo-5H-dibenz[b,d]azepin-7-yl]amino]-1-methyl-2-oxoethyl]-3,5-difluorobenzeneacetamide
Purity:	≥98% (HPLC)
Molecular Weight:	463.48

View Full Technical Details for DBZ

Technical Data
Citations / Reviews (1)

Biological Activity for DBZ

DBZ is an inhibitor of γ-secretase aspartyl protease. DBZ blocks amyloid precursor protein-like (APPL) and Notch cleavage (IC₅₀ values are 2.6 nM and 1.7-2.9 nM, respectively). By inhibiting Notch and the Notch pathway, DBZ improves efficiency of iPSC generation and production of iPSCs from mouse and human keratinocytes without KLF4 and CMYC. In an animal model of Alzheimer's disease, DBZ inhibits the cleavage of amyloid precursor protein into Aβ40. DBZ promotes cochlear supporting cell proliferation and hair cell mitotic regeneration in neonatal mice. DBZ in combination with iDOT1L enables maintenance of human naïve-state pluripotency in non-hypoxic conditions.

DBZ synthesized to Ancillary Material Grade also available.

Compound Libraries for DBZ

DBZ is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for DBZ

M. Wt	463.48
Formula	C₂₆H₂₃F₂N₃O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	209984-56-5
PubChem ID	11454028
InChi Key	QSHGISMANBKLQL-OWJWWREXSA-N
Smiles	CN1C2=C(C=CC=C2)C3=C(C=CC=C3)[C@H](NC([C@@H](NC(CC4=CC(F)=CC(F)=C4)=O)C)=O)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DBZ

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	46.35	100

Preparing Stock Solutions for DBZ

The following data is based on the product molecular weight 463.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.16 mL	10.79 mL	21.58 mL
5 mM	0.43 mL	2.16 mL	4.32 mL
10 mM	0.22 mL	1.08 mL	2.16 mL
50 mM	0.04 mL	0.22 mL	0.43 mL

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References for DBZ

References are publications that support the biological activity of the product.

Isono The combination of dibenzazepine and a DOT1L inhibitor enables a stable maintenance of human naïve-state pluripotency in non-hypoxic conditions. Regen.Ther. 2020 PMID: 33426214
Wu Dibenzazepine promotes cochlear supporting cell proliferation and hair cell regeneration in neonatal mice. Cell Prolif. 2020 PMID: 32677724
van Es Notch/γ-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells. Nature 2005 PMID: 15959515
Ichida Notch inhibition allows oncogene-independent generation of iPS cells. Nat.Chem.Biol. 2014 PMID: 24952596
Groth Pharmacological analysis of Drosophila melanogaster γ-secretase with respect to differential proteolysis of Notch and APP. Mol.Pharmacol. 2010 PMID: 20064975

DBZ