DBIA

Name: DBIA
Brand: Tocris Bioscience
SKU: 8142

Tocris Bioscience, part of Bio-Techne | Catalog # 8142

COA / SDS

Chemoproteomic reagent, binds with reactive cysteines and labels with desthiobiotin

Key Product Details

Description:	Chemoproteomic reagent, binds with reactive cysteines and labels with desthiobiotin
Chemical Name:	(4R,5S)-N-[2-[(2-Iodoacetyl)amino]ethyl]-5-methyl-2-oxo-4-imidazolidinehexanamide
Purity:	≥95% (HPLC)
Molecular Weight:	424.28

View Full Technical Details for DBIA

Technical Data
Citations

Biological Activity for DBIA

DBIA (desthiobiotin iodoacetamide) is a chemoproteomic reagent. DBIA is a cysteine reactive probe used to label and purify proteins by attaching desthiobiotin to cysteine residues in the protein of interest. DBIA can be used for targeted protein labeling and purification studies.

Technical Data for DBIA

M. Wt	424.28
Formula	C₁₄H₂₅IN₄O₃
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	2924824-04-2
PubChem ID	166642567
InChi Key	ASKKBTGJVCEXOA-WDEREUQCSA-N
Smiles	ICC(NCCNC(CCCCC[C@@H]1[C@@H](NC(N1)=O)C)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DBIA

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	42.43	100

Preparing Stock Solutions for DBIA

The following data is based on the product molecular weight 424.28.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.36 mL	11.78 mL	23.57 mL
5 mM	0.47 mL	2.36 mL	4.71 mL
10 mM	0.24 mL	1.18 mL	2.36 mL
50 mM	0.05 mL	0.24 mL	0.47 mL

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Reconstitution Calculator

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References for DBIA

References are publications that support the biological activity of the product.

Kuljanin Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat.Biotechnol. 2021 PMID: 33398154