CT-4 New

Name: CT-4
Brand: Tocris Bioscience
SKU: 8024

Tocris Bioscience, part of Bio-Techne | Catalog # 8024

COA / SDS

Potent and selective HDAC8 degrader (PROTAC®)

Key Product Details

Description:	Potent and selective HDAC8 degrader (PROTAC^®)
Chemical Name:	3-((4-((8-((2-(2,6-Dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)octyl)carbamoyl)benzyl)oxy)-N-hydroxy-4-methoxybenzamide
Purity:	≥98% (HPLC)
Molecular Weight:	699.76

View Full Technical Details for CT-4

Technical Data
Citations

Biological Activity for CT-4

CT-4 is a potent and selective histone deacetylase 8 (HDAC8) degrader (PROTAC^®) (DC₅₀ = 1.8 nM after 24 h, D_max 97%). Comprises a HDAC8-binding moiety joined by a linker to pomalidomide as the ligand for cereblon (CRBN) protein. Displays 20-fold selectivity over HDAC6 (DC₅₀ = 38 nM after 24 h, D_max 69%) and it does not degrade other HDACs.

Displays significant effect on metastasis of MDA-MB-231 cells, and induces degradation and cell death in T-cell leukemia Jurkat cells. Outperformed HDAC8 inhibitors in oncology models. HDAC8 mediates signalling via scaffolding functions in HDAC8/p53/fusion protein complexes, also interacts with STAT3, cAMP and DEC1 via scaffolding functions.

PROTAC^® is a registered trademark of Arvinas Operations, Inc., and is used under license

Technical Data for CT-4

M. Wt	699.76
Formula	C₃₇H₄₁N₅O₉
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	3057302-21-0
PubChem ID	171378332
InChi Key	ARLOQCPNXYNVDU-UHFFFAOYSA-N
Smiles	ONC(C1=CC(OCC2=CC=C(C=C2)C(NCCCCCCCCNC3=CC=CC(C(N4C5CCC(NC5=O)=O)=O)=C3C4=O)=O)=C(C=C1)OC)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CT-4

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	69.98	100

Preparing Stock Solutions for CT-4

The following data is based on the product molecular weight 699.76.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.43 mL	7.15 mL	14.29 mL
5 mM	0.29 mL	1.43 mL	2.86 mL
10 mM	0.14 mL	0.71 mL	1.43 mL
50 mM	0.03 mL	0.14 mL	0.29 mL

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References for CT-4

References are publications that support the biological activity of the product.

Zhao Discovery of highly potent HDAC8 PROTACs with anti-tumor activity. Bioorg.Chem. 2023 PMID: 37098288