CHIR 98014

Name: CHIR 98014
Brand: Tocris Bioscience
SKU: 6695

Tocris Bioscience, part of Bio-Techne | Catalog # 6695

Citations (12)

COA / SDS

Highly potent and selective GSK-3 inhibitor

Key Product Details

Description:	Highly potent and selective GSK-3 inhibitor
Chemical Name:	N⁶-[2-[[4-(2,4-Dichlorophenyl)-5-(1H-imidazol-1-yl)-2-pyrimidinyl]amino]ethyl]-3-nitro-2,6-pyridinediamine
Purity:	≥98% (HPLC)
Molecular Weight:	486.31

View Full Technical Details for CHIR 98014

Technical Data
Citations

Biological Activity for CHIR 98014

CHIR 98014 is a highly potent and selective GSK-3 inhibitor (IC₅₀ values are 0.58 and 0.65 nM for the β and α isoforms, respectively). Exhibits >500-fold selectivity for GSK-3β over a range of other kinases. Improves insulin sensitivity of muscle strips from diabetic rats in vitro and decreases insulin and glucose levels in the oral glucose tolerance test in vivo. Activates Wnt signaling in human adipose stem cells (hASC) and promotes the production of definitive endoderm. Has been used in protocols to derive epicardial cells from hPSCs.

Technical Data for CHIR 98014

M. Wt	486.31
Formula	C₂₀H₁₇Cl₂N₉O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	252935-94-7
PubChem ID	53396311
InChi Key	MDZCSIDIPDZWKL-UHFFFAOYSA-N
Smiles	NC1=NC(NCCNC2=NC=C(C(C3=C(C=C(C=C3)Cl)Cl)=N2)N4C=CN=C4)=CC=C1[N+]([O-])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CHIR 98014

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	9.73	20 with gentle warming

Preparing Stock Solutions for CHIR 98014

The following data is based on the product molecular weight 486.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	10.28 mL	51.41 mL	102.82 mL
1 mM	2.06 mL	10.28 mL	20.56 mL
2 mM	1.03 mL	5.14 mL	10.28 mL
10 mM	0.21 mL	1.03 mL	2.06 mL

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References for CHIR 98014

References are publications that support the biological activity of the product.

Paik and Wu Simply derived epicardial cells. Nat.Biomed.Eng. 2017 PMID: 29354320
Huang Activation of Wnt/β-catenin signalling via GSK3 inhibitors direct differentiation of human adipose stem cells into functional hepatocytes. Sci.Rep. 2017 PMID: 28094799
Ring Selective glycogen synthase kinase 3 inhibitors potentiate Ins activation of glucose transport and utilization in vitro and in vivo. Diabetes 2003 PMID: 12606497