Bz 423

Name: Bz 423
Brand: Tocris Bioscience
SKU: 5791

Tocris Bioscience, part of Bio-Techne | Catalog # 5791

Citations (4)

COA / SDS

ATP synthase inhibitor; proapoptotic

Key Product Details

Description:	ATP synthase inhibitor; proapoptotic
Chemical Name:	7-Chloro-1,3-dihydro-5-(4-hydroxyphenyl)-1-methyl-3-(2-naphthalenylmethyl)-2H-1,4-benzodiazepin-2-one
Purity:	≥98% (HPLC)
Molecular Weight:	440.92

View Full Technical Details for Bz 423

Technical Data
Citations

Biological Activity for Bz 423

Bz 423 is an ATP synthase inhibitor (IC₅₀ ~5 μM). Binds the oligomycin sensitivity conferring protein (OSCP) component of mitochondrial F₁F₀-ATPase in vitro resulting in generation of superoxide and apoptosis. Kills primary B and T cell in mice in vivo. Suppresses autoimmunity and prolongs survival in mouse lupus model.

Technical Data for Bz 423

M. Wt	440.92
Formula	C₂₇H₂₁ClN₂O₂
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	216691-95-1
PubChem ID	5497206
InChi Key	IJEUIRDNQUQMFU-UHFFFAOYSA-N
Smiles	CN1C2=CC=C(C=C2C(C3=CC=C(C=C3)O)=NC(C1=O)CC4=CC5=CC=CC=C5C=C4)Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Bz 423

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	44.09	100
	ethanol	44.09	100

Preparing Stock Solutions for Bz 423

The following data is based on the product molecular weight 440.92.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.27 mL	11.34 mL	22.68 mL
5 mM	0.45 mL	2.27 mL	4.54 mL
10 mM	0.23 mL	1.13 mL	2.27 mL
50 mM	0.05 mL	0.23 mL	0.45 mL

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References for Bz 423

References are publications that support the biological activity of the product.

Blatt Bz-423 superoxide signals B cell apoptosis via Mcl-1, Bak, and Bax. Biochem.Pharmacol. 2009 PMID: 19481066
Blatt Benzodiazepine-induced superoxide signals B cell apoptosis: mechanistic insight and potential therapeutic utility. J.Clin.Invest. 2002 PMID: 12393848
Johnson Identification and validation of the mitochondrial F1F0-ATPase as the molecular target of the immunomodulatory benzodiazepine Bz-423. Chem.Biol. 2005 PMID: 15850986