BRD 0539

Name: BRD 0539
Brand: Tocris Bioscience
SKU: 7153

Tocris Bioscience, part of Bio-Techne | Catalog # 7153

Citations (2)

COA / SDS

Cell permeable and reversible Cas9 inhibitor

Key Product Details

Description:	Cell permeable and reversible Cas9 inhibitor
Chemical Name:	(3aR,4R,9bR)-8-(2-Fluorophenyl)-2,3,3a,4,5,9b-hexahydro-1-[(4-methylphenyl)sulfonyl]-1H-pyrrolo[3,2-c]quinoline-4-methanol
Purity:	≥98% (HPLC)
Molecular Weight:	452.54

View Full Technical Details for BRD 0539

Technical Data
Citations

Biological Activity for BRD 0539

BRD 0539 is a cell-permeable and reversible SpCas9 (Streptococcus pyogenes Cas9) inhibitor. BRD 0539 shows dose-dependent SpCas9 inhibition in an in vitro DNA cleavage assay (apparent IC₅₀ = 22 μM) and in an eGFP disruption assay (apparent EC₅₀ = 11 μM). Allows dose and temporal control of SpCas9-based systems.

Licensing Information

Sold under licence from the Broad Institute

Technical Data for BRD 0539

M. Wt	452.54
Formula	C₂₅H₂₅FN₂O₃S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1403838-79-8
PubChem ID	54666326
InChi Key	CZOIXFMISSBIJL-DCEDVJGZSA-N
Smiles	[H][C@]12CCN([C@@]1([H])C1=CC(=CC=C1N[C@H]2CO)C1=C(F)C=CC=C1)[S](=O)(=O)C1=CC=C(C)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BRD 0539

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	45.25	100
	ethanol	45.25	100

Preparing Stock Solutions for BRD 0539

The following data is based on the product molecular weight 452.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.21 mL	11.05 mL	22.10 mL
5 mM	0.44 mL	2.21 mL	4.42 mL
10 mM	0.22 mL	1.10 mL	2.21 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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References for BRD 0539

References are publications that support the biological activity of the product.

Maji A high-throughput platform to identify small-molecule inhibitors of CRISPR-Cas9. Cell 2019 PMID: 31051099