BPTU

Tocris Bioscience, part of Bio-Techne | Catalog # 6078

COA / SDS

P2Y1 allosteric antagonist

Key Product Details

Description:	P2Y₁ allosteric antagonist
Chemical Name:	N-[2-[2-(1,1-Dimethylethyl)phenoxy]-3-pyridinyl]-N'-[4-(trifluoromethoxy)phenyl]urea
Purity:	≥98% (HPLC)
Molecular Weight:	445.43

View Full Technical Details for BPTU

Technical Data
Citations

Biological Activity for BPTU

BPTU is an allosteric antagonist of P2Y₁ (EC₅₀ = 0.06-0.3 μM). Non-nucleotide ligand. Binds receptor outside of the helical bundle. Blocks inhibition of spontaneous contraction of rat and mouse colon induced by electrical field stimulation, nicotine and P2Y agonists. Antithrombotic; reduces platelet aggregation.

Technical Data for BPTU

M. Wt	445.43
Formula	C₂₃H₂₂F₃N₃O₃
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	870544-59-5
PubChem ID	11510579
InChi Key	AHFLGPTXSIRAQK-UHFFFAOYSA-N
Smiles	CC(C)(C1=C(C=CC=C1)OC2=C(C=CC=N2)NC(NC3=CC=C(C=C3)OC(F)(F)F)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BPTU

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	44.54	100
	ethanol	44.54	100

Preparing Stock Solutions for BPTU

The following data is based on the product molecular weight 445.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.25 mL	11.23 mL	22.45 mL
5 mM	0.45 mL	2.25 mL	4.49 mL
10 mM	0.22 mL	1.12 mL	2.25 mL
50 mM	0.04 mL	0.22 mL	0.45 mL

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Reconstitution Calculator

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References for BPTU

References are publications that support the biological activity of the product.

Zhang Two disparate ligand-binding sites in the human P2Y1 receptor. Nature. 2015 PMID: 25822790
Mañé BPTU, an allosteric antagonist of P2Y1 receptor, blocks nerve mediated inhibitory neuromuscular responses in the gastrointestinal tract of rodents. Neuropharmacology. 2016 PMID: 27496690
Chao Discovery of 2-(phenoxypyridine)-3-phenylureas as small molecule P2Y1 antagonists. J.Med.Chem. 2013 PMID: 23368907