Bleomycin sulfate

Name: Bleomycin sulfate
Brand: Tocris Bioscience
SKU: 7147

Tocris Bioscience, part of Bio-Techne | Catalog # 7147

COA / SDS

DNA synthesis inhibitor. Induces pulmonary inflammation and fibrosis

Key Product Details

Description:	DNA synthesis inhibitor. Induces pulmonary inflammation and fibrosis
Alternative Names:	NSC125066
Purity:	≥90% (HPLC)
Molecular Weight:	1512.6

View Full Technical Details for Bleomycin sulfate

Technical Data
Citations

Biological Activity for Bleomycin sulfate

Bleomycin sulfate is a glycopeptide antibiotic produced by Streptomyces verticillus. It inhibits DNA synthesis and causes single and double-strand breaks at specific base sequences in intracellular DNA of bacterial and mammalian cells. It also acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis. It is an anticancer agent for squamous cell carcinomas (SCC) with IC₅₀ = 4 nM in UT-SCC-19A cells.

In vivo, Bleomycin sulfate induces pulmonary inflammation and fibrosis in lung injury models (mice).

Bleomycin sulfate contains two main components:

1) Bleomycin A₂ hemisulfate, C₅₅H₈₄N₁₇O₂₁S₃.HSO₄, molecular weight = 1512.62 Da

2) Bleomycin B₂, C₅₅H₈₄N₂₀O₂₁S₂, molecular weight = 1425.52 Da.

The exact proportions vary from batch to batch and can be found on the relevant certificate of analysis.

Technical Data for Bleomycin sulfate

M. Wt	1512.6
Formula	C₅₅H₈₄N₁₇O₂₁S₃.HSO₄ (for A₂)
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	9041-93-4
PubChem ID	9812642
InChi Key	WUIABRMSWOKTOF-OYALTWQYSA-N
Smiles	O=S(O)([O-])=O.O=C(N[C@@H]([C@@H](O[C@H]1[C@@H](O[C@@H]2[C@@H](O)[C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O2)[C@@H](O)[C@H](O)[C@H](CO)O1)C3=CN=CN3)C(N[C@@H]([C@H]([C@@H](C(N[C@H](C(NCCC4=NC(C5=NC(C(NCCC[S+](C)C)=O)=CS5)=CS4)=O)[C@@H](C)O)=O)C)O)C)=O)C6=NC([C@@H](NC[C@@H](C(N)=O)N)CC(N)=O)=NC(N)=C6C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Bleomycin sulfate

	Solvent	Max Conc. mg/mL
Solubility
	DMSO	20
	water	20

Preparing Stock Solutions for Bleomycin sulfate

The following data is based on the product molecular weight 1512.6.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.14 mM	4.72 mL	23.61 mL	47.22 mL
0.7 mM	0.94 mL	4.72 mL	9.44 mL
1.4 mM	0.47 mL	2.36 mL	4.72 mL
7 mM	0.09 mL	0.47 mL	0.94 mL

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References for Bleomycin sulfate

References are publications that support the biological activity of the product.

Banerjee Characterization of lung stem cell niches in a mouse model of bleomycin-induced fibrosis. Stem Cell Res.Ther. 2012 PMID: 22643035
Jääskelä-Saari Squamous cell cancer cell lines: sensitivity to bleomycin and suitability for animal xenograft studies. Acta Oto-Laryngologica 1997 PMID: 9288321
Liu Telomere shortening activates TGF-β/Smads signaling in lungs and enhances both lipopolysaccharide and bleomycin-induced pulmonary fibrosis. Pharmacol.Sin 2018 PMID: 29925920
Lin Expression of human carcinoembryonic antigen-related cell adhesion molecule 6 and alveolar progenitor cells in normal and injured lungs of transgenic mice. Physiol.Rep 2015 PMID: 26702074
D'Andrea Sequence specific cleavage of DNA by the antitumor antibiotics neocarzinostatin and bleomycin. Proc.Natl.Acad.Sci.USA 1978 PMID: 80799