AZ1 USP25/28 inhibitor
Tocris Bioscience, part of Bio-Techne | Catalog # 7845
Selective USP25 and USP28 inhibitor
Key Product Details
Description
Selective USP25 and USP28 inhibitor
Product Description
AZ1 USP25/28 inhibitor is a selective inhibitor of USP28 (IC50 = 0.6 μM, Kd= 0.2 - 3.7 μM) and USP25 (IC50 = 0.7 μM). AZ1 USP25/28 inhibitor exhibits selectivity for USP25 and USP28 over other DUB family members. The compound dose-dependently reduces c-Myc levels in colon carcinoma cells in vitro and induces cell death (EC50 values are 18 - 20 μM). In a mouse Alzheimer's disease model, AZ1 USP25/28 inhibitor reduces amyloid burden, attenuates microglial activation and improves synaptic and cognitive function.Product Specifications
Molecular Weight
422.21
Formula
C17H16BrF4NO2
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
2-[[[5-Bromo-2-[[4-fluoro-3-(trifluoromethyl)phenyl]methoxy]phenyl]methyl]amino]ethanol
CAS Number
2165322-94-9
PubChem ID
135397656
InChI Key
ITHSFXDGKQYOED-UHFFFAOYSA-N
SMILES
FC1=CC=C(COC2=CC=C(C=C2CNCCO)Br)C=C1C(F)(F)F
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 42.22 | 100 |
Preparing Stock Solutions
for AZ1 USP25/28 inhibitor
The following data is based on the product molecular weight 422.21
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.37 mL | 11.84 mL | 23.68 mL |
| 5 mM | 0.47 mL | 2.37 mL | 4.74 mL |
| 10 mM | 0.24 mL | 1.18 mL | 2.37 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.47 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Zheng Trisomy 21-induced dysregulation of microglial homeostasis in Alzheimer's brains is mediated by USP25. Sci.Adv. 2021 PMID: 33523861
- Zheng USP25 inhibition ameliorates Alzheimer's pathology through the regulation of APP processing and Aβ generation. J.Clin.Invest. 2022 PMID: 35229730
- Wrigley Identification and characterization of dual inhibitors of the USP25/28 deubiquitinating enzyme subfamily. ACS Chem.Biol. 2017 PMID: 29131570
Product Specific Notices for AZ1 USP25/28 inhibitor
For research use only
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