AZ 3146

Name: AZ 3146
Brand: Tocris Bioscience
SKU: 3994

Tocris Bioscience, part of Bio-Techne | Catalog # 3994

Citations (52)

COA / SDS

Potent and selective Mps1 kinase inhibitor

Key Product Details

Description:	Potent and selective Mps1 kinase inhibitor
Chemical Name:	9-Cyclopentyl-2-[[2-methoxy-4-[(1-methylpiperidin-4-yl)oxy]-phenyl]amino]-7-methyl-7,9-dihydro-8H-purin-8-one
Purity:	≥98% (HPLC)
Molecular Weight:	452.55

View Full Technical Details for AZ 3146

Technical Data
Citations

Biological Activity for AZ 3146

AZ 3146 is a potent and selective monopolar spindle 1 (Mps1) kinase inhibitor (IC₅₀ = 35 nM). Displays selectivity over 46 other kinases including Cdk1 and aurora kinase B. Interferes with chromosome alignment and overrides spindle assembly checkpoint. Inhibits the recruitment of Mad1, Mad2 and centromere protein E (CENP-E) to kinetochores.

Licensing Information

Sold for research purposes only under agreement from AstraZeneca UK Ltd.

Compound Libraries for AZ 3146

AZ 3146 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for AZ 3146

M. Wt	452.55
Formula	C₂₄H₃₂N₆O₃
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1124329-14-1
PubChem ID	56973724
InChi Key	YUKWVHPTFRQHMF-UHFFFAOYSA-N
Smiles	CN(C1=CN=C(NC4=CC=C(OC5CCN(C)CC5)C=C4OC)N=C1N2C3CCCC3)C2=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AZ 3146

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	11.31	25
	ethanol	33.94	75

Preparing Stock Solutions for AZ 3146

The following data is based on the product molecular weight 452.55.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.75 mM	2.95 mL	14.73 mL	29.46 mL
3.75 mM	0.59 mL	2.95 mL	5.89 mL
7.5 mM	0.29 mL	1.47 mL	2.95 mL
37.5 mM	0.06 mL	0.29 mL	0.59 mL

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References for AZ 3146

References are publications that support the biological activity of the product.

Lan and Cleveland A chemical tool box defines mitotic and interphase roles for Mps1 kinase. J.Cell.Biol. 2010 PMID: 20624898
Maciejowski Mps1 directs the assembly of Cdc20 inhbitory complexes during interphase and mitosis to control M phase timing and spindle checkpoint signaling. J.Cell Biol. 2010 PMID: 20624902
Hewitt Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex. J.Cell Biol. 2010 PMID: 20624899