AY 9944 dihydrochloride

Name: AY 9944 dihydrochloride
Brand: Tocris Bioscience
SKU: 1639

Tocris Bioscience, part of Bio-Techne | Catalog # 1639

Citations (6)

COA / SDS

Inhibitor of Hedgehog (Hh) signaling. Inhibits Δ7-dehydrocholesterol reductase

Key Product Details

Description:	Inhibitor of Hedgehog (Hh) signaling. Inhibits Δ⁷-dehydrocholesterol reductase
Chemical Name:	trans-N,N-bis[2-Chlorophenylmethyl]-1,4-cyclohexanedimethanamine dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	464.3

View Full Technical Details for AY 9944 dihydrochloride

Technical Data
Citations

Biological Activity for AY 9944 dihydrochloride

AY 9944 dihydrochloride is an inhibitor of hedgehog (Hh) signaling, possibly via several mechanisms. Inhibits Δ⁷-dehydrocholesterol reductase (IC₅₀ = 13 nM), thus reduces cholesterol biosynthesis, and also inhibits cholesterol esterification. May also directly block the cellular response to Hh proteins. Teratogenic in vivo.

Technical Data for AY 9944 dihydrochloride

M. Wt	464.3
Formula	C₂₂H₂₈Cl₂N₂.2HCl
Storage	Desiccate at RT
Purity	≥98% (HPLC)
CAS Number	366-93-8
PubChem ID	9954082
InChi Key	GZFYZYBWLCYBMI-MYZJJQSMSA-N
Smiles	ClC(C=CC=C3)=C3CNC[C@H]1CC[C@H](CNCC2=C(Cl)C=CC=C2)CC1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AY 9944 dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	23.21	50
	DMSO	2.32	5

Preparing Stock Solutions for AY 9944 dihydrochloride

The following data is based on the product molecular weight 464.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.31 mL	21.54 mL	43.08 mL
2.5 mM	0.86 mL	4.31 mL	8.62 mL
5 mM	0.43 mL	2.15 mL	4.31 mL
25 mM	0.09 mL	0.43 mL	0.86 mL

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References for AY 9944 dihydrochloride

References are publications that support the biological activity of the product.

Moebius Molecular cloning and expression of the human Δ7-sterol reductase. Proc.Natl.Acad.Sci.U.S.A. 1998 PMID: 9465114
Incardona and Eaton Cholesterol in signal transduction. Curr.Opin.Cell Biol. 2000 PMID: 10712926
Kraml Inhibition of the conversion of 7-dehydrocholesterol to cholesterol by AY-9944. Biochem.Biophys.Res.Commun. 1964 PMID: 4283982
Cooper Teratogen-mediated inhibition of target tissue response to Shh signaling. Science 1998 PMID: 9616123