AP 18

Name: AP 18
Brand: Tocris Bioscience
SKU: 3296

Tocris Bioscience, part of Bio-Techne | Catalog # 3296

Citations (17)

COA / SDS

Reversible TRPA1 channel blocker

Key Product Details

Description:	Reversible TRPA1 channel blocker
Chemical Name:	4-(4-Chlorophenyl)-3-methyl-3-buten-2-one oxime
Purity:	≥98% (HPLC)
Molecular Weight:	209.67

View Full Technical Details for AP 18

Technical Data
Citations

Biological Activity for AP 18

AP 18 is a reversible TRPA1 channel blocker (IC₅₀ values are 3.1 and 4.5 μM at human and mouse TRPA1 respectively). Blocks cinnamaldehyde-induced but not capsaicin-induced nociception and reverses mechanical hyperalgesia in vivo. Also blocks TRPA1 pore dilation (IC₅₀ = 10.3 μM for the inhibition of Yo-Pro uptake).

Compound Libraries for AP 18

AP 18 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for AP 18

M. Wt	209.67
Formula	C₁₁H₁₂ClNO
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	55224-94-7
PubChem ID	9584673
InChi Key	MHTJEUOFLVQMCL-NJHPPEEMSA-N
Smiles	ClC1=CC=C(/C=C(C)/C(C)=N/O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AP 18

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	20.97	100
	ethanol	20.97	100

Preparing Stock Solutions for AP 18

The following data is based on the product molecular weight 209.67.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.77 mL	23.85 mL	47.69 mL
5 mM	0.95 mL	4.77 mL	9.54 mL
10 mM	0.48 mL	2.38 mL	4.77 mL
50 mM	0.10 mL	0.48 mL	0.95 mL

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References for AP 18

References are publications that support the biological activity of the product.

Chen Pore dilation occurs in TRPA1 but not in TRPM8 channels. Mol.Pain 2009 PMID: 19159452
Taylor-Clark Nitrooleic acid, an endogenous product of nitrative stress, activates nociceptive sensory nerves via the direct activation of TRPA1. Mol.Pharmacol. 2009 PMID: 19171673
Petrus A role of TRPA1 in mechanical hyperalgesia is revealed by pharmacological inhibition. Mol.Pain 2007 PMID: 18086313