Anisomycin
Tocris Bioscience, part of Bio-Techne | Catalog # 1290
JNK, SAPK and p38 activator
Product Description
Anisomycin is a protein synthesis inhibitor (blocks translation). Potent activator of stress-activated protein kinases (JNK/SAPK) and p38 MAP kinase. Acts as a potent signaling agonist to selectively elicit homologous desensitization of immediate early gene induction (c-fos, fosB, c-jun, junB and junD).Product Specifications
Molecular Weight
265.31
Formula
C14H19NO4
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
(2R,3S,4S)-2-[(4-Methoxyphenyl)methyl]-3,4-pyrrolidinediol 3-acetate
CAS Number
22862-76-6
PubChem ID
253602
InChI Key
YKJYKKNCCRKFSL-RDBSUJKOSA-N
SMILES
O[C@H]1CN[C@H](CC2=CC=C(OC)C=C2)[C@@H]1OC(C)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 26.53 | 100 | |
| Ethanol | 13.27 | 50 |
Preparing Stock Solutions
for Anisomycin
The following data is based on the product molecular weight 265.31
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.77 mL | 18.85 mL | 37.69 mL |
| 5 mM | 0.75 mL | 3.77 mL | 7.54 mL |
| 10 mM | 0.38 mL | 1.88 mL | 3.77 mL |
| 50 mM | 0.08 mL | 0.38 mL | 0.75 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Sanchez Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun. Nature 1994 PMID: 7997269
- Kyriakis The stress-activated protein kinase subfamily of c-Jun kinases. Nature 1994 PMID: 8177321
- Croons The protein synthesis inhibitor anisomycin induces macrophage apoptosis in rabbit atherosclerotic plaques through p38 mitogen-activated protein kinase. J.Pharmacol.Exp.Ther. 2009 PMID: 19286921
- Hazzalin Anisomycin selectively desensitizes signalling components involved in stress kinase activation and fos and jun induction. Mol.Cell.Biol. 1998 PMID: 9528756
- Cano Anisomycin-activated protein kinases p45 and p55 but not mitogen-activated protein kinases ERK-1 and -2 are implicated in the induction of c-fos and c-jun. Mol.Cell.Biol. 1994 PMID: 7935449
Product Specific Notices for Anisomycin
For research use only
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