AMZ 30

Name: AMZ 30
Brand: Tocris Bioscience
SKU: 6923

Tocris Bioscience, part of Bio-Techne | Catalog # 6923

COA / SDS

Potent, selective and irreversible protein phosphatase methylesterase-1 (PME-1) inhibitor

Key Product Details

Description:	Potent, selective and irreversible protein phosphatase methylesterase-1 (PME-1) inhibitor
Chemical Name:	(2E)-2-[(4-Fluorophenyl)sulfonyl]-3-[1-[(3-nitrophenyl)sulfonyl]-1H-pyrrol-2-yl]-2-propenenitrile
Purity:	≥98% (HPLC)
Molecular Weight:	461.45

View Full Technical Details for AMZ 30

Technical Data
Citations

Biological Activity for AMZ 30

AMZ 30 is a potent, selective and irreversible inhibitor of protein phosphatase methylesterase-1 (PME-1; IC₅₀ = 600 nM) with > 100-fold selectivity for PME-1 over other serine hydrolases in human cell lysates. AMZ 30 inactivates PME-1 and reduces the demethylated form of serine/threonine protein phosphatase 2A (PP2A) in HEK 293T cells. AMZ 30 perturbs leucine carboxyl methyltransferase-1 (LCMT1)-PME-1 methylation equilibrium leading to shortening of mitotic spindles and mitotic arrest in HeLa cells. It also shows antiproliferative properties in an in vitro model of endometrial carcinoma.

Technical Data for AMZ 30

M. Wt	461.45
Formula	C₁₉H₁₂FN₃O₆S₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1313613-09-0
PubChem ID	44607965
InChi Key	GUCUORSUHTZMBW-YBFXNURJSA-N
Smiles	C(=C(/S(=O)(=O)C1=CC=C(F)C=C1)\C#N)\C=2N(S(=O)(=O)C3=CC(N(=O)=O)=CC=C3)C=CC2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AMZ 30

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	46.15	100

Preparing Stock Solutions for AMZ 30

The following data is based on the product molecular weight 461.45.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.17 mL	10.84 mL	21.67 mL
5 mM	0.43 mL	2.17 mL	4.33 mL
10 mM	0.22 mL	1.08 mL	2.17 mL
50 mM	0.04 mL	0.22 mL	0.43 mL

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References for AMZ 30

References are publications that support the biological activity of the product.

Xia A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size. Cell Cycle 2015 PMID: 25839665
Bachovchin Discovery and optimization of sulfonyl acrylonitriles as selective, covalent inhibitors of protein phosphatase methylesterase-1. J.Med.Chem. 2011 PMID: 21639134
Elgenaidi Regulation of the phosphoprotein phosphatase 2A system and its modulation during oxidative stress: A potential therapeutic target? Pharmacol.Ther. 2019 PMID: 30797822