AF-DX 116

Name: AF-DX 116
Brand: Tocris Bioscience
SKU: 1105

Tocris Bioscience, part of Bio-Techne | Catalog # 1105

Citations (70)

COA / SDS

Selective M2 antagonist

Key Product Details

Description:	Selective M₂ antagonist
Alternative Names:	Otenzepad
Chemical Name:	11-[[2-[(Diethylamino)methyl]-1-piperidinyl]acetyl]-5,11-dihydro-6H-pyrido[2,3-b][1,4]benzodiazepin-6-one
Purity:	≥98% (HPLC)
Molecular Weight:	421.54

View Full Technical Details for AF-DX 116

Technical Data
Citations

Biological Activity for AF-DX 116

AF-DX 116 is a selective M₂ muscarinic receptor antagonist. K_i values are 64, 417, 786, 211 and 5130 nM for human recombinant M₂, M₁, M₃, M₄ and M₅ muscarinic receptors, respectively.

Licensing Information

Sold with the permission of Boehringer Ingelheim GmbH

Technical Data for AF-DX 116

M. Wt	421.54
Formula	C₂₄H₃₁N₅O₂
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	102394-31-0
PubChem ID	107867
InChi Key	UBRKDAVQCKZSPO-UHFFFAOYSA-N
Smiles	CCN(CC)CC1CCCCN1CC(=O)N1C2=C(C=CC=C2)C(=O)NC2=CC=CN=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AF-DX 116

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	10.54	25 with gentle warming

Preparing Stock Solutions for AF-DX 116

The following data is based on the product molecular weight 421.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.25 mM	9.49 mL	47.45 mL	94.89 mL
1.25 mM	1.90 mL	9.49 mL	18.98 mL
2.5 mM	0.95 mL	4.74 mL	9.49 mL
12.5 mM	0.19 mL	0.95 mL	1.90 mL

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References for AF-DX 116

References are publications that support the biological activity of the product.

Mansfield Muscarinic receptor subtypes in human bladder detrusor and mucosa, studied by radioligand binding and quantitative competitive RT-PCR: changes in ageing. Br.J.Pharmacol. 2005 PMID: 15723094
Eglen Muscarinic receptor subtypes and smooth muscle function. Pharmacol.Rev. 1996 PMID: 8981565
Hammer Binding profile of a novel cardioselective muscarinic receptor antagonist, AF-DX 116, to membranes of peripheral tissues and brain in the rat. Life Sci. 1986 PMID: 3754610
Billard Identification of the primary muscarinic autoreceptor subtype in rat striatum as M₂ through a correlation of in vivo microdialysis and in vitro receptor binding data. J.Pharmacol.Exp.Ther. 1995 PMID: 7714776