Ac₄ManNAz

Product Description

Ac₄ManNAz is a cell-permeable, unnatural azide-containing monosaccharide building block. Ac₄ManNAz is metabolized and incorporated as azidosialic acid into cell surface and secreted sialoglycoproteins to allow Cu(I)-free click chemistry for further conjugation or detection. Ac₄ManNAz can be used for bioorthogonal labeling of human prostate tissue slice cultures; the target sialoglycoproteins can be biotinylated for proteomic analysis or reacted with a cyclooctyne-functionalized optical probe for imaging. Ac₄ManNAz is also used to label glycoRNAs on the cell surface.

Solubility

Preparing Stock Solutions for Ac₄ManNAz

The following data is based on the product molecular weight 430.37

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.32 mL	11.62 mL	23.24 mL
5 mM	0.46 mL	2.32 mL	4.65 mL
10 mM	0.23 mL	1.16 mL	2.32 mL
50 mM	0.05 mL	0.23 mL	0.46 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Zhu Coupling aptamer-based protein tagging with metabolic glycan labeling for in situ visualization and biological function study of exosomal protein-specific glycosylation. Angew.Chem.Int.Ed.Engl. 2021 PMID: 34043264
• Shajahan Carbohydrate-neuroactive hybrid strategy for metabolic glycan engineering of the central nervous system in vivo. J.Am.Chem.Soc. 2017 PMID: 27997162
• Flynn Small RNAs are modified with N-glycans and displayed on the surface of living cells. Cell 2021 PMID: 34004145
• Spiciarich Bioorthogonal labeling of human prostate cancer tissue slice cultures for glycoproteomics. Angew.Chem.Int.Ed.Engl. 2017 PMID: 28649697