AC 5216

Name: AC 5216
Brand: Tocris Bioscience
SKU: 5281

Tocris Bioscience, part of Bio-Techne | Catalog # 5281

Citations (2)

COA / SDS

Selective TSPO ligand

Key Product Details

Description:	Selective TSPO ligand
Alternative Names:	XBD173
Chemical Name:	N-Ethyl-7,8-dihydro-7-methyl-8-oxo-2-phenyl-N-(phenylmethyl)-9H-purine-9-acetamide
Purity:	≥98% (HPLC)
Molecular Weight:	401.46

View Full Technical Details for AC 5216

Technical Data
Citations

Biological Activity for AC 5216

AC 5216 is a ligand of translocator protein (TSPO, peripheral benzodiazepine receptor). Exhibits high affinity for TSPO in rat whole brain (K_i = 0.297 nM), and high potency against human and rat glial TSPO (IC₅₀ values are 2.73 and 3.04 nM respectively). Displays no noticeable binding to a number of other receptors, transporters or ion channels. Also displays anxiolytic and antidepressant effects in rodent models, without inducing benzodiazepine-like adverse effects.

Compound Libraries for AC 5216

AC 5216 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for AC 5216

M. Wt	401.46
Formula	C₂₃H₂₃N₅O₂
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	226954-04-7
PubChem ID	6433109
InChi Key	NBMBIEOUVBHEBM-UHFFFAOYSA-N
Smiles	O=C(N1C)N(CC(N(CC)CC2=CC=CC=C2)=O)C3=C1C=NC(C4=CC=CC=C4)=N3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AC 5216

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	40.15	100

Preparing Stock Solutions for AC 5216

The following data is based on the product molecular weight 401.46.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.49 mL	12.45 mL	24.91 mL
5 mM	0.50 mL	2.49 mL	4.98 mL
10 mM	0.25 mL	1.25 mL	2.49 mL
50 mM	0.05 mL	0.25 mL	0.50 mL

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Reconstitution Calculator

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References for AC 5216

References are publications that support the biological activity of the product.

Qiu Repeated administration of AC-5216, a ligand for the 18kDa translocator protein, improves behavioral deficits in a mouse model of post-traumatic stress disorder. Prog.Neuropsychopharmacol.Biol.Psychiatry 2013 PMID: 23624119
Kita Antianxiety and antidepressant-like effects of AC-5216, a novel mitochondrial benzodiazepine receptor ligand. Br.J.Pharmacol. 2004 PMID: 15249420