A 196
Tocris Bioscience, part of Bio-Techne | Catalog # 6167
Potent and selective SUV420H1/H2 inhibitor
Key Product Details
Description
Potent and selective SUV420H1/H2 inhibitor
Product Description
A 196 is a potent and selective SUV420H1/H2 inhibitor (IC50 values are 25 and 144 nM respectively) that exhibits 100-fold selectivity over other histone methyltransferases and non-epigenetic targets. A 196 inhibits the di- and tri-methylation of H4K20me in multiple cell lines (IC50 < 1 μM).To request the negative control for A 196, please fill out the SGC 2043 request form on the SGC website.
Licensing Information
This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the A-196 probe summary on the SGC website.External Portal Information
Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of A196 is reviewed on the Chemical Probes website.Product Specifications
Molecular Weight
359.25
Formula
C18H16Cl2N4
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
6,7-Dichloro-N-cyclopentyl-4-(4-pyridinyl)-1-phthalazinamine
CAS Number
1982372-88-2
PubChem ID
117072548
InChI Key
ABGOSOMRWSYAOB-UHFFFAOYSA-N
SMILES
ClC1=CC2=C(C(C3=CC=NC=C3)=NN=C2NC4CCCC4)C=C1Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.18 | 20 |
Preparing Stock Solutions
for A 196
The following data is based on the product molecular weight 359.25
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 13.92 mL | 69.59 mL | 139.18 mL |
| 1 mM | 2.78 mL | 13.92 mL | 27.84 mL |
| 2 mM | 1.39 mL | 6.96 mL | 13.92 mL |
| 10 mM | 0.28 mL | 1.39 mL | 2.78 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Scheer A chemical biology toolbox to study protein methyltransferases and epigenetic signaling. Nat.Commun. 2019 PMID: 30604761
- Brustel Histone H4K20 tri-methylation at late-firing origins ensures timely heterochromatin replication. EMBO J 2017 PMID: 28778956
- Bromberg The SUV4-20 inhibitor A-196 verifies a role for epigenetics in genomic integrity. Nat.Chem.Biol. 2017 PMID: 28114273
Product Specific Notices for A 196
For research use only
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