8-CPT-2Me-cAMP, sodium salt
Tocris Bioscience, part of Bio-Techne | Catalog # 1645
Selective Epac activator
Product Description
8-CPT-2Me-cAMP, sodium salt is a selective activator of Epac, the cAMP-sensitive guanine nucleotide-exchange factor for Rap1 and Rap2. Activates Epac1 (EC50 = 2.2 μM), but not PKA (EC50 > 10 μM). Stimulates Epac-mediated Ca2+-mediated Ca2+ release in pancreatic β-cells in vitro. Cell permeable analog (Cat. No. 4853) also available.Licensing Information
Sold with the permission of BioLog Life Science InstituteProduct Specifications
Molecular Weight
507.82
Formula
C17H16ClN5O6PS.Na
Storage
Store at -20°C
Purity
≥99% (HPLC)
Chemical Name
8-(4-Chlorophenylthio)-2'-O-methyladenosine-3',5'-cyclic monophosphate sodium salt
CAS Number
634207-53-7
PubChem ID
16759604
InChI Key
VHTMKTNUXDACHB-KHXPSBENSA-M
SMILES
[Na+].[H][C@@]12COP([O-])(=O)O[C@@]1([H])[C@@H](OC)[C@@H](O2)N1C(SC2=CC=C(Cl)C=C2)=NC2=C1N=CN=C2N
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 50.78 | 100 |
Preparing Stock Solutions
for 8-CPT-2Me-cAMP, sodium salt
The following data is based on the product molecular weight 507.82
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.97 mL | 9.85 mL | 19.69 mL |
| 5 mM | 0.39 mL | 1.97 mL | 3.94 mL |
| 10 mM | 0.20 mL | 0.98 mL | 1.97 mL |
| 50 mM | 0.04 mL | 0.20 mL | 0.39 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Kang Epac-selective cAMP analog 8-pCPT-2'-O-Me-cAMP as a stimulus for Ca2+-induced Ca2+ release and exocytosis in pancreatic βcells. J.Biol.Chem. 2003 PMID: 12496249
- Kawasaki A family of cAMP-binding proteins that directly activate Rap1. Science 1998 PMID: 9856955
- Enserink A novel Epac-specific cAMP analogue demonstrates independent regulation of Rap1 and ERK. Nat.Cell.Biol. 2002 PMID: 12402047
Product Specific Notices for 8-CPT-2Me-cAMP, sodium salt
For research use only
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