8-CPT-2Me-cAMP, sodium salt

Name: 8-CPT-2Me-cAMP, sodium salt
Brand: Tocris Bioscience
SKU: 1645

Tocris Bioscience, part of Bio-Techne | Catalog # 1645

Citations (18)

COA / SDS

Selective Epac activator

Key Product Details

Description:	Selective Epac activator
Chemical Name:	8-(4-Chlorophenylthio)-2'-O-methyladenosine-3',5'-cyclic monophosphate sodium salt
Purity:	≥99% (HPLC)
Molecular Weight:	507.82

View Full Technical Details for 8-CPT-2Me-cAMP, sodium salt

Technical Data
Citations

Biological Activity for 8-CPT-2Me-cAMP, sodium salt

8-CPT-2Me-cAMP, sodium salt is a selective activator of Epac, the cAMP-sensitive guanine nucleotide-exchange factor for Rap1 and Rap2. Activates Epac1 (EC₅₀ = 2.2 μM), but not PKA (EC₅₀ > 10 μM). Stimulates Epac-mediated Ca²⁺-mediated Ca²⁺ release in pancreatic β-cells in vitro. Cell permeable analog (Cat. No. 4853) also available.

Licensing Information

Sold with the permission of BioLog Life Science Institute

Technical Data for 8-CPT-2Me-cAMP, sodium salt

M. Wt	507.82
Formula	C₁₇H₁₆ClN₅O₆PS.Na
Storage	Store at -20°C
Purity	≥99% (HPLC)
CAS Number	634207-53-7
PubChem ID	16759604
InChi Key	VHTMKTNUXDACHB-KHXPSBENSA-M
Smiles	[Na+].[H][C@@]12COP([O-])(=O)O[C@@]1([H])[C@@H](OC)[C@@H](O2)N1C(SC2=CC=C(Cl)C=C2)=NC2=C1N=CN=C2N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 8-CPT-2Me-cAMP, sodium salt

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	50.78	100

Preparing Stock Solutions for 8-CPT-2Me-cAMP, sodium salt

The following data is based on the product molecular weight 507.82.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.97 mL	9.85 mL	19.69 mL
5 mM	0.39 mL	1.97 mL	3.94 mL
10 mM	0.20 mL	0.98 mL	1.97 mL
50 mM	0.04 mL	0.20 mL	0.39 mL

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References for 8-CPT-2Me-cAMP, sodium salt

References are publications that support the biological activity of the product.

Kang Epac-selective cAMP analog 8-pCPT-2'-O-Me-cAMP as a stimulus for Ca²⁺-induced Ca²⁺ release and exocytosis in pancreatic βcells. J.Biol.Chem. 2003 PMID: 12496249
Kawasaki A family of cAMP-binding proteins that directly activate Rap1. Science 1998 PMID: 9856955
Enserink A novel Epac-specific cAMP analogue demonstrates independent regulation of Rap1 and ERK. Nat.Cell.Biol. 2002 PMID: 12402047