Lysis Kit - RIPA Buffer for Charge Assays
ProteinSimple, part of Bio-Techne | Catalog # CBS401
Compatible with Peggy Sue and NanoPro 1000 systems
Key Product Details
Features
Kit includes RIPA Lysis Buffer (10 mL), Aqueous Protease Inhibitor (400 µL) and DMSO Inhibitor Mix (200 µL).
Product Benefits
- RIPA Buffer contains, 20 mM HEPES (pH 7.5), 150 mM NaCl, 1% NP-40, 0.25% Sodium Deoxycholate, 10% Glycerol, 1X DMSO and aqueous inhibitor mix.
- Aqueous Protease Inhibitor contains 40mM NaF, 12 mM beta-glycerophosphate, 1 mM Sodium Orthovanadate, 0.5mM EDTA, 2.5 mM EGTA
- DMSO Inhibitor Mix contains 1mM AEBSF, 5µg/ml Aprotinin, 50 µM Bestatin, 5µg/ml E-64 Protease Inhibitor, 10 µg/ml Leupeptin, 7µg/mL Pepstatin A, Phosphatase inhibitor cocktail (with effective concentrations of Cantharidin, Bromotetramisole and Microcystin LR)
Compatible Instruments
NanoPro 1000 - Simple Western charge-based assays | ProteinSimple
NanoPro 1000 from ProteinSimple, a Bio-Techne brand, characterizes post-translational protein modifications. See beyond what's detected by a traditional Western Blot.
Peggy Sue - Automated Western Blot System | ProteinSimple
Peggy Sue from ProteinSimple, a Bio-Techne brand, automates western blotting technology. Run 96 data points in one overnight run with as little as 5 µL of sample.
Preparation and Storage
Shipping
The product is shipped in multiple packages at different temperatures.
Storage
Please see the package insert for the recommended storage of each component.
Loading...
Loading...